Analysis of unintegrated avian RNA tumor virus double stranded DNA intermediates

T. W. Hsu, J. L. Sabran, G. E. Mark, Ramareddy Guntaka, J. M. Taylor

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Previous studies by Guntaka et al. have shown that the unintegrated DNA intermediates of avian RNA tumor virus replication can be readily isolated from cultures of the quail tumor line QT-6 at 1 day after infection. The intermediates include double-stranded linear and covalently closed circular DNA species. Using the analysis procedure of Southern together with previously obtained information regarding the sites of action of certain restriction endonucleases on avian sarcoma virus DNA, we have further characterized the viral DNA intermediates. Evidence is presented that, relative to the RNA genome, most of the linear species possess a direct terminal sequence redundancy equivalent to 0.5 x 10 6 ± 0.3 x 10 6 daltons of double-stranded DNA. Some of the circular forms also possess a sequence redundancy of 0.21 x 10 6 ± 0.03 x 10 6 daltons.

Original languageEnglish (US)
Pages (from-to)810-818
Number of pages9
JournalJournal of Virology
Volume28
Issue number3
StatePublished - Dec 1 1978
Externally publishedYes

Fingerprint

dsDNA viruses
Retroviridae
RNA
neoplasms
DNA
Avian Sarcoma Viruses
Circular DNA
Quail
DNA Restriction Enzymes
Viral DNA
circular DNA
Rous sarcoma virus
Virus Replication
restriction endonucleases
virus replication
quails
Genome
Infection
genome
Neoplasms

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Hsu, T. W., Sabran, J. L., Mark, G. E., Guntaka, R., & Taylor, J. M. (1978). Analysis of unintegrated avian RNA tumor virus double stranded DNA intermediates. Journal of Virology, 28(3), 810-818.

Analysis of unintegrated avian RNA tumor virus double stranded DNA intermediates. / Hsu, T. W.; Sabran, J. L.; Mark, G. E.; Guntaka, Ramareddy; Taylor, J. M.

In: Journal of Virology, Vol. 28, No. 3, 01.12.1978, p. 810-818.

Research output: Contribution to journalArticle

Hsu, TW, Sabran, JL, Mark, GE, Guntaka, R & Taylor, JM 1978, 'Analysis of unintegrated avian RNA tumor virus double stranded DNA intermediates', Journal of Virology, vol. 28, no. 3, pp. 810-818.
Hsu, T. W. ; Sabran, J. L. ; Mark, G. E. ; Guntaka, Ramareddy ; Taylor, J. M. / Analysis of unintegrated avian RNA tumor virus double stranded DNA intermediates. In: Journal of Virology. 1978 ; Vol. 28, No. 3. pp. 810-818.
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