Angiotensin II induces phosphorylation of eukaryotic protein synthesis initiation factor 4E in vascular smooth muscle cells

Rao Gadiparthi, Kathy K. Griendling, Robert M. Frederickson, Nahum Sonenberg, R. Wayne Alexander

Research output: Contribution to journalArticle

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Abstract

Angiotensin II has been shown to induce hypertrophy of cultured vascular smooth muscle cells (VSMC). To understand the mechanisms of induction of the hypertrophy, we studied its effect on the phosphorylation state of eIF-4E, a rate-limiting eukaryotic protein synthesis initiation factor whose activity has been shown to be regulated by phosphorylation. Angiotensin II induced a 2-3-fold increase in the phosphorylation of eIF-4E in VSMC. The stimulation of phosphorylation was apparent at 20 min and persisted for at least 12 h. Phosphoamino acid analysis revealed that serine is the major residue of eIF- 4E phosphorylated by angiotensin II. Staurosporine and calphostin C, two potent inhibitors of the serine/threonine protein kinase, protein kinase C, significantly attenuated the angiotensin II-induced eIF-4E phosphorylation. Staurosporine and calphostin C also blunted the angiotensin II-stimulated protein synthesis. Together, these observations indicate that angiotensin II induces phosphorylation of eIF-4E in a protein kinase C-dependent manner and suggest that this pathway may play an important role in the mechanism by which angiotensin II causes hypertrophy of VSMC.

Original languageEnglish (US)
Pages (from-to)7180-7184
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number10
StatePublished - Mar 11 1994

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Peptide Initiation Factors
Phosphorylation
Vascular Smooth Muscle
Angiotensin II
Smooth Muscle Myocytes
Muscle
Cells
Proteins
Hypertrophy
Staurosporine
Protein Kinase C
Phosphoamino Acids
Protein-Serine-Threonine Kinases
Serine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Angiotensin II induces phosphorylation of eukaryotic protein synthesis initiation factor 4E in vascular smooth muscle cells. / Gadiparthi, Rao; Griendling, Kathy K.; Frederickson, Robert M.; Sonenberg, Nahum; Alexander, R. Wayne.

In: Journal of Biological Chemistry, Vol. 269, No. 10, 11.03.1994, p. 7180-7184.

Research output: Contribution to journalArticle

Gadiparthi, Rao ; Griendling, Kathy K. ; Frederickson, Robert M. ; Sonenberg, Nahum ; Alexander, R. Wayne. / Angiotensin II induces phosphorylation of eukaryotic protein synthesis initiation factor 4E in vascular smooth muscle cells. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 10. pp. 7180-7184.
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