Anisotropic regulation of Ankrd2 gene expression in skeletal muscle by mechanical stretch

Junaith Mohamed, Michael A. Lopez, Gregory A. Cox, Aladin M. Boriek

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The diaphragm muscles in vivo are subjected to mechanical forces both in the direction of the muscle fibers and in the direction transverse to the fibers. However, the effect of directional mechanical forces in skeletal muscle gene regulation is completely unknown. Here, we identified that stretch in the longitudinal and transverse directions to the diaphragm muscle fibers up-regulated Ankrd2 gene expression by two distinct signaling pathways in wild-type (WT) and mdm, a mouse model of muscular dystrophy with early-onset of progressive muscle-wasting. Stretch in the longitudinal direction activated both NF-κB and AP-1 transcription factors, whereas stretch in the transverse direction activated only AP-1 transcription factor. Interestingly, longitudinal stretch activated Ankrd2 promoter only by NF-κB, whereas transverse stretch activated Ankrd2 promoter by AP-1. Moreover, we found that longitudinal stretch activated Akt, which up-regulated Ankrd2 expression through NF-κB. However, transverse stretch activated Ras-GTP, Raf-1, and Erk1/2 proteins, which up-regulated Ankrd2 expression through AP-1. Surprisingly, the stretch-activated NF-κB and AP-1 signaling pathways was not involved in Ankrd2 regulation at the basal level, which was high in the mdm mouse diaphragm. Taken together, our data show the anisotropic regulation of Ankrd2 gene expression in the diaphragm muscles of WT and mdm mice via two distinct mechanosensitive signaling pathways.

Original languageEnglish (US)
Pages (from-to)3330-3340
Number of pages11
JournalFASEB Journal
Volume24
Issue number9
DOIs
StatePublished - Sep 1 2010
Externally publishedYes

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Transcription Factor AP-1
Gene Expression Regulation
Gene expression
Muscle
Diaphragm
Skeletal Muscle
Diaphragms
Muscles
Fibers
Muscular Dystrophies
Guanosine Triphosphate
Direction compound
Gene Expression
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Anisotropic regulation of Ankrd2 gene expression in skeletal muscle by mechanical stretch. / Mohamed, Junaith; Lopez, Michael A.; Cox, Gregory A.; Boriek, Aladin M.

In: FASEB Journal, Vol. 24, No. 9, 01.09.2010, p. 3330-3340.

Research output: Contribution to journalArticle

Mohamed, Junaith ; Lopez, Michael A. ; Cox, Gregory A. ; Boriek, Aladin M. / Anisotropic regulation of Ankrd2 gene expression in skeletal muscle by mechanical stretch. In: FASEB Journal. 2010 ; Vol. 24, No. 9. pp. 3330-3340.
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