Annulus fibrosus tissue engineering using lamellar silk scaffolds

Sang Hyug Park, Eun Seok Gil, Biman B. Mandal, Hongsik Cho, Jonathan A. Kluge, Byoung Hyun Min, David L. Kaplan

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Degeneration of the intervertebral disc (IVD) represents a significant muscular skeletal disease. Recently, scaffolds composed of synthetic, natural and hybrid biomaterials have been investigated as options to restore the IVD; however, they lack the hallmark lamellar morphological features of annulus fibrosus (AF) tissue. The goal of regenerating the disc is to achieve anatomical morphology as well as restoration of mechanical and biological function. In this study, two types of scaffold morphology formed from silk fibroin were investigated towards the goal of AF tissue restoration. The first design mimics the lamellar features of the IVD that are associated with the AF region. The second is a porous spongy scaffold that serves as a control. Toroidal scaffolds were formed from the lamellar and porous silk material systems to generate structures with an outer diameter of 8mm, inner diameter of 3.5mm and a height of 3mm. The inter-lamellar spacing in the lamellar scaffold was 150-250μm and the average pore sizes in the porous scaffolds were 100-250μm. The scaffolds were seeded with porcine AF cells and, after growth over defined time frames in vitro, histology, biochemical assays, mechanical testing and gene expression indicated that the lamellar scaffold generated results that were more favourable in terms of ECM expression and tissue function than the porous scaffold for AF tissue. Further, the seeded porcine AF cells supported the native shape of AF tissue in the lamellar silk scaffolds. The lamellar silk scaffolds were effective in the formation of AF-like tissue in vitro.

Original languageEnglish (US)
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume6
Issue numberSUPPL. 3
DOIs
StatePublished - Dec 1 2012

Fingerprint

Silk
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Tissue
Intervertebral Disc
Swine
Fibroins
Restoration
Intervertebral Disc Degeneration
Annulus Fibrosus
Biocompatible Materials
Muscular Diseases
Military electronic countermeasures
Histology
Mechanical testing
Gene expression
Biomaterials
Pore size

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Annulus fibrosus tissue engineering using lamellar silk scaffolds. / Park, Sang Hyug; Gil, Eun Seok; Mandal, Biman B.; Cho, Hongsik; Kluge, Jonathan A.; Min, Byoung Hyun; Kaplan, David L.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 6, No. SUPPL. 3, 01.12.2012.

Research output: Contribution to journalArticle

Park, Sang Hyug ; Gil, Eun Seok ; Mandal, Biman B. ; Cho, Hongsik ; Kluge, Jonathan A. ; Min, Byoung Hyun ; Kaplan, David L. / Annulus fibrosus tissue engineering using lamellar silk scaffolds. In: Journal of Tissue Engineering and Regenerative Medicine. 2012 ; Vol. 6, No. SUPPL. 3.
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