Anterior versus posterior intraocular melanoma metastatic differences in a murine model

H. E. Grossniklaus, Matthew Wilson, B. C. Barron, M. J. Lynn

Research output: Contribution to journalArticle

Abstract

Purpose. We evaluated the utility of a murine model to study differences in metastatic rate of anterior versus posterior ocular melanoma. Methods. Twenty-nine 12-week old C57BL6 mice were inoculated into the anterior chamber (AC) or posterior compartment (PC) of the right eye with 5×105/5μl tissue culture Queens melanoma cells. The right eye was enucleated at 14 days post-inoculation with a necropsy performed. The eye was evaluated for presence of melanoma and the mean of the ten largest nucleoli (MLN) in tumor cells was measured with a digital Filar micrometer. The number of metastases was determined at necropsy. Results. Melanoma grew in 12 of 18 AC mice (66%) and 9 of 11 PC mice (82%). The metastatic rate was significantly smaller for AC tumors (33%) versus PC tumors (89%) (p=.02). All AC and no PC melanomas that metastasized to the lungs metastasized to ipsilateral cervical lymph nodes. The median number of metastases per tumor was significantly smaller for AC tumors compared to PC tumors (p=.01). There was a median of 0 metastases (range 0 to 14) per AC tumor and 4 metastases (range 0 to 38) per PC tumor. There was no significant difference of the MLN for AC versus PC melanoma (p=.215). The mean MLN of melanomas that did and did not metastasize was 3.12 μm for each. Conclusions. Posterior ocular melanoma metastasizes more often than anterior ocular melanoma in this murine model, similar to human ocular melanoma.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

Melanoma
Anterior Chamber
Neoplasms
Neoplasm Metastasis
Lymph Nodes
Lung

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Anterior versus posterior intraocular melanoma metastatic differences in a murine model. / Grossniklaus, H. E.; Wilson, Matthew; Barron, B. C.; Lynn, M. J.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

@article{107eb9ac38a146b2baa4235d1c5ce39f,
title = "Anterior versus posterior intraocular melanoma metastatic differences in a murine model",
abstract = "Purpose. We evaluated the utility of a murine model to study differences in metastatic rate of anterior versus posterior ocular melanoma. Methods. Twenty-nine 12-week old C57BL6 mice were inoculated into the anterior chamber (AC) or posterior compartment (PC) of the right eye with 5×105/5μl tissue culture Queens melanoma cells. The right eye was enucleated at 14 days post-inoculation with a necropsy performed. The eye was evaluated for presence of melanoma and the mean of the ten largest nucleoli (MLN) in tumor cells was measured with a digital Filar micrometer. The number of metastases was determined at necropsy. Results. Melanoma grew in 12 of 18 AC mice (66{\%}) and 9 of 11 PC mice (82{\%}). The metastatic rate was significantly smaller for AC tumors (33{\%}) versus PC tumors (89{\%}) (p=.02). All AC and no PC melanomas that metastasized to the lungs metastasized to ipsilateral cervical lymph nodes. The median number of metastases per tumor was significantly smaller for AC tumors compared to PC tumors (p=.01). There was a median of 0 metastases (range 0 to 14) per AC tumor and 4 metastases (range 0 to 38) per PC tumor. There was no significant difference of the MLN for AC versus PC melanoma (p=.215). The mean MLN of melanomas that did and did not metastasize was 3.12 μm for each. Conclusions. Posterior ocular melanoma metastasizes more often than anterior ocular melanoma in this murine model, similar to human ocular melanoma.",
author = "Grossniklaus, {H. E.} and Matthew Wilson and Barron, {B. C.} and Lynn, {M. J.}",
year = "1996",
month = "2",
day = "15",
language = "English (US)",
volume = "37",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Anterior versus posterior intraocular melanoma metastatic differences in a murine model

AU - Grossniklaus, H. E.

AU - Wilson, Matthew

AU - Barron, B. C.

AU - Lynn, M. J.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. We evaluated the utility of a murine model to study differences in metastatic rate of anterior versus posterior ocular melanoma. Methods. Twenty-nine 12-week old C57BL6 mice were inoculated into the anterior chamber (AC) or posterior compartment (PC) of the right eye with 5×105/5μl tissue culture Queens melanoma cells. The right eye was enucleated at 14 days post-inoculation with a necropsy performed. The eye was evaluated for presence of melanoma and the mean of the ten largest nucleoli (MLN) in tumor cells was measured with a digital Filar micrometer. The number of metastases was determined at necropsy. Results. Melanoma grew in 12 of 18 AC mice (66%) and 9 of 11 PC mice (82%). The metastatic rate was significantly smaller for AC tumors (33%) versus PC tumors (89%) (p=.02). All AC and no PC melanomas that metastasized to the lungs metastasized to ipsilateral cervical lymph nodes. The median number of metastases per tumor was significantly smaller for AC tumors compared to PC tumors (p=.01). There was a median of 0 metastases (range 0 to 14) per AC tumor and 4 metastases (range 0 to 38) per PC tumor. There was no significant difference of the MLN for AC versus PC melanoma (p=.215). The mean MLN of melanomas that did and did not metastasize was 3.12 μm for each. Conclusions. Posterior ocular melanoma metastasizes more often than anterior ocular melanoma in this murine model, similar to human ocular melanoma.

AB - Purpose. We evaluated the utility of a murine model to study differences in metastatic rate of anterior versus posterior ocular melanoma. Methods. Twenty-nine 12-week old C57BL6 mice were inoculated into the anterior chamber (AC) or posterior compartment (PC) of the right eye with 5×105/5μl tissue culture Queens melanoma cells. The right eye was enucleated at 14 days post-inoculation with a necropsy performed. The eye was evaluated for presence of melanoma and the mean of the ten largest nucleoli (MLN) in tumor cells was measured with a digital Filar micrometer. The number of metastases was determined at necropsy. Results. Melanoma grew in 12 of 18 AC mice (66%) and 9 of 11 PC mice (82%). The metastatic rate was significantly smaller for AC tumors (33%) versus PC tumors (89%) (p=.02). All AC and no PC melanomas that metastasized to the lungs metastasized to ipsilateral cervical lymph nodes. The median number of metastases per tumor was significantly smaller for AC tumors compared to PC tumors (p=.01). There was a median of 0 metastases (range 0 to 14) per AC tumor and 4 metastases (range 0 to 38) per PC tumor. There was no significant difference of the MLN for AC versus PC melanoma (p=.215). The mean MLN of melanomas that did and did not metastasize was 3.12 μm for each. Conclusions. Posterior ocular melanoma metastasizes more often than anterior ocular melanoma in this murine model, similar to human ocular melanoma.

UR - http://www.scopus.com/inward/record.url?scp=33750195668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750195668&partnerID=8YFLogxK

M3 - Article

VL - 37

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -