Anti-amyloidogenic activity of IgGs contained in normal plasma

Brian O'Nuallain, Angela D. Williams, Helen P. McWilliams-Koeppen, Luis Acero, Alfred Weber, Hartmut Ehrlich, Hans P. Schwarz, Alan Solomon

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Introduction We have previously shown that a subpopulation of naturally occurring human IgGs has therapeutic potential for the amyloid-associated disorders. These molecules cross-react with conformational epitopes on amyloidogenic assemblies, including amyloid beta (Aβ) protein fibrils that are a pathological hallmark of Alzheimer's disease. Materials and Methods Using our europium-linked immunosorbant assay, we established that ~95% of 260 screened donor plasma samples had amyloid fibril-reactive IgGs and Aβ conformer-reactive IgGs with minimal binding to Aβ monomers. Anti-amyloidogenic reactivity was diverse and attributed to Aβ targeting multiple fibril-related binding sites and/or variations in multidentate binding. Results and Discussion There was no correlation between anti-fibril and anti-oligomer reactivity and donor age (19 to 60 years old) or gender. These findings demonstrate the inherent but diverse anti-amyloidogenic activity of natural IgGs contained in normal plasma. Conclusion Our studies provide support for investigating the clinical significance and physiological function of this novel class of antibodies.

Original languageEnglish (US)
JournalJournal of Clinical Immunology
Volume30
Issue numberSUPPL. 1
DOIs
StatePublished - May 1 2010

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Amyloid
Europium
Serum Amyloid A Protein
Immunoglobulin Isotypes
Amyloid beta-Peptides
Epitopes
Alzheimer Disease
Binding Sites
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

O'Nuallain, B., Williams, A. D., McWilliams-Koeppen, H. P., Acero, L., Weber, A., Ehrlich, H., ... Solomon, A. (2010). Anti-amyloidogenic activity of IgGs contained in normal plasma. Journal of Clinical Immunology, 30(SUPPL. 1). https://doi.org/10.1007/s10875-010-9413-6

Anti-amyloidogenic activity of IgGs contained in normal plasma. / O'Nuallain, Brian; Williams, Angela D.; McWilliams-Koeppen, Helen P.; Acero, Luis; Weber, Alfred; Ehrlich, Hartmut; Schwarz, Hans P.; Solomon, Alan.

In: Journal of Clinical Immunology, Vol. 30, No. SUPPL. 1, 01.05.2010.

Research output: Contribution to journalArticle

O'Nuallain, B, Williams, AD, McWilliams-Koeppen, HP, Acero, L, Weber, A, Ehrlich, H, Schwarz, HP & Solomon, A 2010, 'Anti-amyloidogenic activity of IgGs contained in normal plasma', Journal of Clinical Immunology, vol. 30, no. SUPPL. 1. https://doi.org/10.1007/s10875-010-9413-6
O'Nuallain B, Williams AD, McWilliams-Koeppen HP, Acero L, Weber A, Ehrlich H et al. Anti-amyloidogenic activity of IgGs contained in normal plasma. Journal of Clinical Immunology. 2010 May 1;30(SUPPL. 1). https://doi.org/10.1007/s10875-010-9413-6
O'Nuallain, Brian ; Williams, Angela D. ; McWilliams-Koeppen, Helen P. ; Acero, Luis ; Weber, Alfred ; Ehrlich, Hartmut ; Schwarz, Hans P. ; Solomon, Alan. / Anti-amyloidogenic activity of IgGs contained in normal plasma. In: Journal of Clinical Immunology. 2010 ; Vol. 30, No. SUPPL. 1.
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