Anti-diarrheal constituents of Alpinia oxyphylla

Junqing Zhang, Sheng Wang, Yonghui Li, Peng Xu, Feng Chen, Yinfeng Tan, Jinao Duan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Isolation, screening and in vivo assays have been used for evaluating anti-diarrhea bioactive of Alpinia oxyphylla. Preliminary experimental results showed that 95% ethanol extract and 90% ethanol elution significantly extended the onset time of diarrhea and reduced the wet feces proportion, however 50% ethanol election had no effect on diarrhea. Chemical analysis results displayed that Nootkatone, Tectochrysin and yakuchinone A may be bioactive ingredients for curing diarrhea. Duodenum in vitro experiment showed that Tectochrysin 50, 100 μM reduces carbachol-induced contraction, while yakuchinone A and Nootkatone had no effect. Bioinformatic computational method as molecular docking has been complementary to experimentally work to explore the potential mechanism. The study of pathogenesis of diarrhea in humans and animal models suggested that Na+/H+ exchanger3 (NHE3) and aquaporin4 (AQP4) are causative agents of diarrhea. The analysis was done on the basis of scoring and binding ability and the docking analysis showed that Tectochrysin has maximum potential against NHE3 (PDB ID: 2OCS) and AQP4 (PDB ID: 3GD8). Tectochrysin indicated minimum energy score and the highest number of interactions with active site residues. These results suggested that A. oxyphyllamight exhibit its anti-diarrhea effect partially by affecting the proteins of NHE3 and AQP4 with its active ingredient Tectochrysin.

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalFitoterapia
Volume89
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Alpinia
Diarrhea
Ethanol
Carbachol
Computational Biology
Duodenum
Feces
Catalytic Domain
Animal Models
tectochrysin

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Zhang, J., Wang, S., Li, Y., Xu, P., Chen, F., Tan, Y., & Duan, J. (2013). Anti-diarrheal constituents of Alpinia oxyphylla. Fitoterapia, 89(1), 149-156. https://doi.org/10.1016/j.fitote.2013.04.001

Anti-diarrheal constituents of Alpinia oxyphylla. / Zhang, Junqing; Wang, Sheng; Li, Yonghui; Xu, Peng; Chen, Feng; Tan, Yinfeng; Duan, Jinao.

In: Fitoterapia, Vol. 89, No. 1, 01.01.2013, p. 149-156.

Research output: Contribution to journalArticle

Zhang, J, Wang, S, Li, Y, Xu, P, Chen, F, Tan, Y & Duan, J 2013, 'Anti-diarrheal constituents of Alpinia oxyphylla', Fitoterapia, vol. 89, no. 1, pp. 149-156. https://doi.org/10.1016/j.fitote.2013.04.001
Zhang J, Wang S, Li Y, Xu P, Chen F, Tan Y et al. Anti-diarrheal constituents of Alpinia oxyphylla. Fitoterapia. 2013 Jan 1;89(1):149-156. https://doi.org/10.1016/j.fitote.2013.04.001
Zhang, Junqing ; Wang, Sheng ; Li, Yonghui ; Xu, Peng ; Chen, Feng ; Tan, Yinfeng ; Duan, Jinao. / Anti-diarrheal constituents of Alpinia oxyphylla. In: Fitoterapia. 2013 ; Vol. 89, No. 1. pp. 149-156.
@article{e6965076898a4558987a2e0ba036f6e0,
title = "Anti-diarrheal constituents of Alpinia oxyphylla",
abstract = "Isolation, screening and in vivo assays have been used for evaluating anti-diarrhea bioactive of Alpinia oxyphylla. Preliminary experimental results showed that 95{\%} ethanol extract and 90{\%} ethanol elution significantly extended the onset time of diarrhea and reduced the wet feces proportion, however 50{\%} ethanol election had no effect on diarrhea. Chemical analysis results displayed that Nootkatone, Tectochrysin and yakuchinone A may be bioactive ingredients for curing diarrhea. Duodenum in vitro experiment showed that Tectochrysin 50, 100 μM reduces carbachol-induced contraction, while yakuchinone A and Nootkatone had no effect. Bioinformatic computational method as molecular docking has been complementary to experimentally work to explore the potential mechanism. The study of pathogenesis of diarrhea in humans and animal models suggested that Na+/H+ exchanger3 (NHE3) and aquaporin4 (AQP4) are causative agents of diarrhea. The analysis was done on the basis of scoring and binding ability and the docking analysis showed that Tectochrysin has maximum potential against NHE3 (PDB ID: 2OCS) and AQP4 (PDB ID: 3GD8). Tectochrysin indicated minimum energy score and the highest number of interactions with active site residues. These results suggested that A. oxyphyllamight exhibit its anti-diarrhea effect partially by affecting the proteins of NHE3 and AQP4 with its active ingredient Tectochrysin.",
author = "Junqing Zhang and Sheng Wang and Yonghui Li and Peng Xu and Feng Chen and Yinfeng Tan and Jinao Duan",
year = "2013",
month = "1",
day = "1",
doi = "10.1016/j.fitote.2013.04.001",
language = "English (US)",
volume = "89",
pages = "149--156",
journal = "Fitoterapia",
issn = "0367-326X",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Anti-diarrheal constituents of Alpinia oxyphylla

AU - Zhang, Junqing

AU - Wang, Sheng

AU - Li, Yonghui

AU - Xu, Peng

AU - Chen, Feng

AU - Tan, Yinfeng

AU - Duan, Jinao

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Isolation, screening and in vivo assays have been used for evaluating anti-diarrhea bioactive of Alpinia oxyphylla. Preliminary experimental results showed that 95% ethanol extract and 90% ethanol elution significantly extended the onset time of diarrhea and reduced the wet feces proportion, however 50% ethanol election had no effect on diarrhea. Chemical analysis results displayed that Nootkatone, Tectochrysin and yakuchinone A may be bioactive ingredients for curing diarrhea. Duodenum in vitro experiment showed that Tectochrysin 50, 100 μM reduces carbachol-induced contraction, while yakuchinone A and Nootkatone had no effect. Bioinformatic computational method as molecular docking has been complementary to experimentally work to explore the potential mechanism. The study of pathogenesis of diarrhea in humans and animal models suggested that Na+/H+ exchanger3 (NHE3) and aquaporin4 (AQP4) are causative agents of diarrhea. The analysis was done on the basis of scoring and binding ability and the docking analysis showed that Tectochrysin has maximum potential against NHE3 (PDB ID: 2OCS) and AQP4 (PDB ID: 3GD8). Tectochrysin indicated minimum energy score and the highest number of interactions with active site residues. These results suggested that A. oxyphyllamight exhibit its anti-diarrhea effect partially by affecting the proteins of NHE3 and AQP4 with its active ingredient Tectochrysin.

AB - Isolation, screening and in vivo assays have been used for evaluating anti-diarrhea bioactive of Alpinia oxyphylla. Preliminary experimental results showed that 95% ethanol extract and 90% ethanol elution significantly extended the onset time of diarrhea and reduced the wet feces proportion, however 50% ethanol election had no effect on diarrhea. Chemical analysis results displayed that Nootkatone, Tectochrysin and yakuchinone A may be bioactive ingredients for curing diarrhea. Duodenum in vitro experiment showed that Tectochrysin 50, 100 μM reduces carbachol-induced contraction, while yakuchinone A and Nootkatone had no effect. Bioinformatic computational method as molecular docking has been complementary to experimentally work to explore the potential mechanism. The study of pathogenesis of diarrhea in humans and animal models suggested that Na+/H+ exchanger3 (NHE3) and aquaporin4 (AQP4) are causative agents of diarrhea. The analysis was done on the basis of scoring and binding ability and the docking analysis showed that Tectochrysin has maximum potential against NHE3 (PDB ID: 2OCS) and AQP4 (PDB ID: 3GD8). Tectochrysin indicated minimum energy score and the highest number of interactions with active site residues. These results suggested that A. oxyphyllamight exhibit its anti-diarrhea effect partially by affecting the proteins of NHE3 and AQP4 with its active ingredient Tectochrysin.

UR - http://www.scopus.com/inward/record.url?scp=84885097930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885097930&partnerID=8YFLogxK

U2 - 10.1016/j.fitote.2013.04.001

DO - 10.1016/j.fitote.2013.04.001

M3 - Article

VL - 89

SP - 149

EP - 156

JO - Fitoterapia

JF - Fitoterapia

SN - 0367-326X

IS - 1

ER -