Anti-human type II collagen CD19+ B cells are present in patients with rheumatoid arthritis and healthy individuals

X. He, Andrew Kang, J. M. Stuart

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective. To determine the frequency and repertoire of CD19+ B cells capable of producing antibodies reactive to type II collagen (CII) in synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) and PB of healthy control individuals. Methods. CD19+ B cells were isolated and activated to secrete immunoglobulins (Ig) by CD4+ T cells. Frequencies of anti-CII B cells were determined by limiting dilution analysis. The isotype and cross-reactivity of the antibodies produced were determined by ELISA. Results. SF and PB from 5 patients and PB from 4 healthy controls were analyzed. Anti-CII CD19+ B cells were identified in all samples tested. In the RA SF, the percentage of activated B cells reactive to human CII was significantly higher than in the PB of patients with RA (p <0.05) or controls (p <0.01). A majority of anti-human CII B cells from patients' SF secreted IgG isotype, whereas most anti-human CII B cells in PB of patients and controls secreted IgM. The anti-CII B cells, regardless of source, are usually reactive to both native and denatured human CII, to different types of human collagens, and to type II collagens from different species. Conclusion. Anti-CII CD19+ B cells responsive to activated helper T cells are present in both patients with RA and healthy individuals. However, these B cells, especially those secreting the IgG isotype, accumulate in the inflamed joints of RA patients.

Original languageEnglish (US)
Pages (from-to)2168-2175
Number of pages8
JournalJournal of Rheumatology
Volume28
Issue number10
StatePublished - 2001
Externally publishedYes

Fingerprint

Collagen Type II
Rheumatoid Arthritis
B-Lymphocytes
Synovial Fluid
Immunoglobulin G
Antibodies
Helper-Inducer T-Lymphocytes
Immunoglobulins
Collagen
Joints
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology

Cite this

Anti-human type II collagen CD19+ B cells are present in patients with rheumatoid arthritis and healthy individuals. / He, X.; Kang, Andrew; Stuart, J. M.

In: Journal of Rheumatology, Vol. 28, No. 10, 2001, p. 2168-2175.

Research output: Contribution to journalArticle

@article{374b9b01b10a465da005c50d01b14cda,
title = "Anti-human type II collagen CD19+ B cells are present in patients with rheumatoid arthritis and healthy individuals",
abstract = "Objective. To determine the frequency and repertoire of CD19+ B cells capable of producing antibodies reactive to type II collagen (CII) in synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) and PB of healthy control individuals. Methods. CD19+ B cells were isolated and activated to secrete immunoglobulins (Ig) by CD4+ T cells. Frequencies of anti-CII B cells were determined by limiting dilution analysis. The isotype and cross-reactivity of the antibodies produced were determined by ELISA. Results. SF and PB from 5 patients and PB from 4 healthy controls were analyzed. Anti-CII CD19+ B cells were identified in all samples tested. In the RA SF, the percentage of activated B cells reactive to human CII was significantly higher than in the PB of patients with RA (p <0.05) or controls (p <0.01). A majority of anti-human CII B cells from patients' SF secreted IgG isotype, whereas most anti-human CII B cells in PB of patients and controls secreted IgM. The anti-CII B cells, regardless of source, are usually reactive to both native and denatured human CII, to different types of human collagens, and to type II collagens from different species. Conclusion. Anti-CII CD19+ B cells responsive to activated helper T cells are present in both patients with RA and healthy individuals. However, these B cells, especially those secreting the IgG isotype, accumulate in the inflamed joints of RA patients.",
author = "X. He and Andrew Kang and Stuart, {J. M.}",
year = "2001",
language = "English (US)",
volume = "28",
pages = "2168--2175",
journal = "Journal of Rheumatology",
issn = "0315-162X",
publisher = "Journal of Rheumatology",
number = "10",

}

TY - JOUR

T1 - Anti-human type II collagen CD19+ B cells are present in patients with rheumatoid arthritis and healthy individuals

AU - He, X.

AU - Kang, Andrew

AU - Stuart, J. M.

PY - 2001

Y1 - 2001

N2 - Objective. To determine the frequency and repertoire of CD19+ B cells capable of producing antibodies reactive to type II collagen (CII) in synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) and PB of healthy control individuals. Methods. CD19+ B cells were isolated and activated to secrete immunoglobulins (Ig) by CD4+ T cells. Frequencies of anti-CII B cells were determined by limiting dilution analysis. The isotype and cross-reactivity of the antibodies produced were determined by ELISA. Results. SF and PB from 5 patients and PB from 4 healthy controls were analyzed. Anti-CII CD19+ B cells were identified in all samples tested. In the RA SF, the percentage of activated B cells reactive to human CII was significantly higher than in the PB of patients with RA (p <0.05) or controls (p <0.01). A majority of anti-human CII B cells from patients' SF secreted IgG isotype, whereas most anti-human CII B cells in PB of patients and controls secreted IgM. The anti-CII B cells, regardless of source, are usually reactive to both native and denatured human CII, to different types of human collagens, and to type II collagens from different species. Conclusion. Anti-CII CD19+ B cells responsive to activated helper T cells are present in both patients with RA and healthy individuals. However, these B cells, especially those secreting the IgG isotype, accumulate in the inflamed joints of RA patients.

AB - Objective. To determine the frequency and repertoire of CD19+ B cells capable of producing antibodies reactive to type II collagen (CII) in synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) and PB of healthy control individuals. Methods. CD19+ B cells were isolated and activated to secrete immunoglobulins (Ig) by CD4+ T cells. Frequencies of anti-CII B cells were determined by limiting dilution analysis. The isotype and cross-reactivity of the antibodies produced were determined by ELISA. Results. SF and PB from 5 patients and PB from 4 healthy controls were analyzed. Anti-CII CD19+ B cells were identified in all samples tested. In the RA SF, the percentage of activated B cells reactive to human CII was significantly higher than in the PB of patients with RA (p <0.05) or controls (p <0.01). A majority of anti-human CII B cells from patients' SF secreted IgG isotype, whereas most anti-human CII B cells in PB of patients and controls secreted IgM. The anti-CII B cells, regardless of source, are usually reactive to both native and denatured human CII, to different types of human collagens, and to type II collagens from different species. Conclusion. Anti-CII CD19+ B cells responsive to activated helper T cells are present in both patients with RA and healthy individuals. However, these B cells, especially those secreting the IgG isotype, accumulate in the inflamed joints of RA patients.

UR - http://www.scopus.com/inward/record.url?scp=0034795512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034795512&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 2168

EP - 2175

JO - Journal of Rheumatology

JF - Journal of Rheumatology

SN - 0315-162X

IS - 10

ER -