Anti-infective properties of Lactobacillus fermentum against Staphylococcus aureus and Pseudomonas aeruginosa

Parvathi Varma, N. Nisha, Kavitha R. Dinesh, Anil V. Kumar, Raja Biswas

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Surgical wounds and implant-associated Staphylococcus aureus and Pseudomonas aeruginosa infections are often difficult to treat because of limited susceptibility of several of these strains to conventional antibiotics. As a result, there is a constant need for new alternative drugs. The aim of this study was to investigate the antimicrobial properties of Lactobacillus fermentum, a probiotic bacterium, which we have isolated from colonic biopsies. The inhibition of S. aureus and P. aeruginosa growth was evaluated by coincubating with L. fermentum strains. Growth inhibition was tested for several of their clinical isolates using agar well diffusion assays. For biofilm assay S. aureus and P. aeruginosa were grown on the glass slides and in 96-well plates in presence of 2.5 μg/ml culture filtrate of L. fermentum. Biofilms were photographed using confocal microscope or stained with 0.1% crystal violet. Reduction in the cytotoxicity of S. aureus and P. aeruginosa was observed in presence of 2.5 μg/ml L. fermentum-spent media. Using in vitroexperiments, we showed that L. fermentum-secreted compound(s) inhibits the growth, cytotoxicity and biofilm formation of several S. aureus and P. aeruginosa strains. Compound(s) present in the culture supernatant of L. fermentum may have promising applications in treating hospital-acquired infections.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalJournal of Molecular Microbiology and Biotechnology
Volume20
Issue number3
DOIs
StatePublished - Jul 1 2011

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Lactobacillus fermentum
Pseudomonas aeruginosa
Staphylococcus aureus
Biofilms
Growth
Gentian Violet
Pseudomonas Infections
Probiotics
Cross Infection
Agar
Glass
Anti-Bacterial Agents
Bacteria
Biopsy
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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Anti-infective properties of Lactobacillus fermentum against Staphylococcus aureus and Pseudomonas aeruginosa. / Varma, Parvathi; Nisha, N.; Dinesh, Kavitha R.; Kumar, Anil V.; Biswas, Raja.

In: Journal of Molecular Microbiology and Biotechnology, Vol. 20, No. 3, 01.07.2011, p. 137-143.

Research output: Contribution to journalArticle

Varma, Parvathi ; Nisha, N. ; Dinesh, Kavitha R. ; Kumar, Anil V. ; Biswas, Raja. / Anti-infective properties of Lactobacillus fermentum against Staphylococcus aureus and Pseudomonas aeruginosa. In: Journal of Molecular Microbiology and Biotechnology. 2011 ; Vol. 20, No. 3. pp. 137-143.
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