Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process

W. Chen, S. Oh, A. P. Ong, N. Oh, Y. Liu, Harry Courtney, M. Appleford, J. L. Ong

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Since bacterial infection is a rising complication following the wide use of implant, there is considerable attention on the effect of implant surface properties on bacterial adhesion. In this study, the effect of silver (Ag) doped hydroxyapatite (HA) coatings on initial antibacterial adhesion and osteoblast cell proliferation and differentiation was investigated. Using a sol-gel process, HA coatings doped with 1 wt % AgNO3 (AgHA1.0) and 1.5 wt % Ag (AgHA1.5) were prepared. Coated surfaces were characterized using X-ray diffraction (XRD) and contact angles measurements. The initial bacteria adhesion was evaluated using a RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus, whereas osteoblast proliferation and differentiation were evaluated using human embryonic palatal mesenchyme cells (HEPM), an osteoblast precursor cell line. In this study, XRD analysis of all surfaces indicated peaks corresponding to HA. Contact angles for AgHA surfaces were observed to be significantly lower when compared to HA surfaces. In vitro initial bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on AgHA surfaces when compared to HA surface. The use of HEPM cells indicated no significant difference in double-stranded DNA (dsDNA) production between all surfaces. Additionally, no differences in alkaline phosphatase specific activity were observed between HA and AgHA1.0 surfaces. Overall, it was concluded that AgHA1.0 has the similar biological activity as HA, with respect to bone cell proliferation and differentiation. In addition, the AgHA1.0 was also concluded to have the ability to minimize the initial bacteria adhesion.

Original languageEnglish (US)
Pages (from-to)899-906
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume82
Issue number4
DOIs
StatePublished - Sep 15 2007

Fingerprint

Durapatite
Hydroxyapatite
Silver
Sol-gel process
Coatings
Adhesion
Osteoblasts
Cell proliferation
Contact angle
Bacteria
Phosphatases
Angle measurement
Bioactivity
X ray diffraction analysis
Surface properties
Alkaline Phosphatase
Bone
Cells
X ray diffraction

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process. / Chen, W.; Oh, S.; Ong, A. P.; Oh, N.; Liu, Y.; Courtney, Harry; Appleford, M.; Ong, J. L.

In: Journal of Biomedical Materials Research - Part A, Vol. 82, No. 4, 15.09.2007, p. 899-906.

Research output: Contribution to journalArticle

Chen, W. ; Oh, S. ; Ong, A. P. ; Oh, N. ; Liu, Y. ; Courtney, Harry ; Appleford, M. ; Ong, J. L. / Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process. In: Journal of Biomedical Materials Research - Part A. 2007 ; Vol. 82, No. 4. pp. 899-906.
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