Antibacterial, cytotoxic and antioxidant potential of methanolic extract of phyllanthus acidus L

Md Mominur Rahman, Md Razibul Habib, S M Raquibul Hasan, Mohammed Aktar Sayeed, Md Sohel Rana

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The objective of the present study was to evaluate antimicrobial, cytotoxic and antioxidant potential of methanol extract of fruit part of Phyllanthus acidus. Phytochemical screening showed evidence of glycoside, tannin and resin. Antimicrobial activity was evaluated using disc diffusion method against 13 pathogenic bacteria where in higher concentration the extract showed moderate to good zone of inhibition against B. megaterium and B. subtilis also with S. typhi and S. dysenteriae. In case of brine shrimp cytotoxicity assay, showed good result with LC50 value 4.46 μg/ml where as LC90 value was 70.79 μg/ml. Antioxidant activity was evaluated by using total flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical assay, reducing power assessment, cupric ion reducing capacity assay (CUPRAC method). The extract showed moderate to good antioxidant activity in a dose dependent manner and found to contain flavonoid compounds. In DPPH radical scavenging assay IC50 value found 2063.42 μg/ml and compared to ascorbic acid with 52 μg/ml. The extract also showed good cupric ion reducing capacity. The results of the present study indicate that the extract possesses moderate to good antioxidant potential.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalInternational Journal of Drug Development and Research
Volume3
Issue number2
StatePublished - Apr 1 2011

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Phyllanthus
Antioxidants
Flavonoids
Ions
Artemia
Tannins
Phytochemicals
Glycosides
Ascorbic Acid
Inhibitory Concentration 50
Free Radicals
Methanol
Fruit
Bacteria

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Antibacterial, cytotoxic and antioxidant potential of methanolic extract of phyllanthus acidus L. / Rahman, Md Mominur; Habib, Md Razibul; Hasan, S M Raquibul; Sayeed, Mohammed Aktar; Rana, Md Sohel.

In: International Journal of Drug Development and Research, Vol. 3, No. 2, 01.04.2011, p. 154-161.

Research output: Contribution to journalArticle

Rahman, Md Mominur ; Habib, Md Razibul ; Hasan, S M Raquibul ; Sayeed, Mohammed Aktar ; Rana, Md Sohel. / Antibacterial, cytotoxic and antioxidant potential of methanolic extract of phyllanthus acidus L. In: International Journal of Drug Development and Research. 2011 ; Vol. 3, No. 2. pp. 154-161.
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