Antibiotic-loaded chitosan film for infection prevention

A preliminary in vitro characterization

J. Keaton Smith, Joel D. Bumgardner, Harry Courtney, Mark S. Smeltzer, Warren O. Haggard

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The growing infection rate by methicillin-resistant Staphylococcus aureus, especially with bone fracture fixation implants, is a major concern in extremity musculoskeletal wound treatment. This preliminary investigation evaluates the ability of chitosan film to be loaded with daptomycin and vancomycin in the operating room, in situ loading, and applied to musculoskeletal fixation devices to lessen or prevent infection. Films with 61, 71, and 80% degrees of deacetylation (DDA) made using lactic or acetic acid solvents were analyzed for their antibiotic uptake, elution, and activity along with film swelling ratio, ultimate tensile strength, Young's modulus, adhesive strength, and degradation. Chitosan films after 1 min of rehydration were able in a simulated, clinical setting to maintain mechanical integrity and adhesive strength to be applied to bone fracture fixation devices or implant surfaces. The film percent degradation increased with DDA increasing from 61 to 80%, but film degradation rate decreased in the presence of antibiotics. Eighty percent DDA chitosan films were optimal for absorbing and eluting antibiotics. Antibiotics eluted by the flms were active against Staphylococcus aureus. These findings indicate that an 80% DDA chitosan film is potentially advantageous as a clinically adjunctive treatment in musculoskeletal injuries to lessen or prevent infections.

Original languageEnglish (US)
Pages (from-to)203-211
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume94
Issue number1
DOIs
StatePublished - Jul 1 2010

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Chitosan
Antibiotics
Anti-Bacterial Agents
Fracture fixation
Degradation
Adhesives
Bone
Daptomycin
Operating rooms
Methicillin
Vancomycin
Lactic acid
Acetic acid
Acetic Acid
Swelling
Tensile strength
Elastic moduli

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Antibiotic-loaded chitosan film for infection prevention : A preliminary in vitro characterization. / Smith, J. Keaton; Bumgardner, Joel D.; Courtney, Harry; Smeltzer, Mark S.; Haggard, Warren O.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 94, No. 1, 01.07.2010, p. 203-211.

Research output: Contribution to journalArticle

Smith, J. Keaton ; Bumgardner, Joel D. ; Courtney, Harry ; Smeltzer, Mark S. ; Haggard, Warren O. / Antibiotic-loaded chitosan film for infection prevention : A preliminary in vitro characterization. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2010 ; Vol. 94, No. 1. pp. 203-211.
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