Antibody to Rabbit Macrophage Type V Collagenase/Gelatinise and its Use to Further Characterize the Enzyme

Carlo L. Mainardi, Karen Hasty, Margaret S. Hibbs

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A highly specific antiserum was raised against purified rabbit alveolar macrophage type V collagenase/gelatinise. The reactivity of the antiserum was tested in ELISA and was detected at a high titer. Competitive inhibition ELISA demonstrated a linear inhibition of reactivity by pure antigen if either pure antigen or crude culture medium was used as the solid phase antigen. In the latter case,>90% inhibition was achieved indicating a high degree of specificity of the antibody. Immobilized antibody was successful in absorbing the type V collagenolytic activity. Immunoblot analysis of crude culture medium revealed a single immunoreactive band which correlated with both the stained gel of the purified antigen as well as the only zone of gelatinolysis in a gelatin/acrylamide gel. This monospecific antibody will be a useful tool in the further characterization of this enzyme as well as the delineation of its relative role in chronic inflammation.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalTopics in Catalysis
Volume4
Issue number3
DOIs
StatePublished - Jan 1 1984

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Collagenases
Macrophages
Rabbits
Antigens
Antibodies
Enzymes
Culture Media
Immune Sera
Gels
Enzyme-Linked Immunosorbent Assay
Immobilized Antibodies
Antibody Specificity
Acrylamide
Alveolar Macrophages
Gelatin
Inflammation

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Rheumatology

Cite this

Antibody to Rabbit Macrophage Type V Collagenase/Gelatinise and its Use to Further Characterize the Enzyme. / Mainardi, Carlo L.; Hasty, Karen; Hibbs, Margaret S.

In: Topics in Catalysis, Vol. 4, No. 3, 01.01.1984, p. 209-217.

Research output: Contribution to journalArticle

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