Antioxidant and antihyperglycemic activities of methanolic extract of Glinus oppositifolius leaves

Nazia Hoque, Mohammad Zafar Imam, Saleha Akter, Jamiuddin Ahmed, Ehsanul Hoque Mazumder, S M Raquibul Hasan, Sohel Rana

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The present study was carried out to investigate the antioxidant and antidiabetic potential of methanolic extract of Glinus oppositifolius leaves. The antioxidant potential was examined by DPPH radical scavenging assay, nitric oxide (NO) scavenging assay, determination of total flavonoid content and total antioxidant capacity assay. Antihyperglycemic activity was studied by oral glucose tolerance test (OGTT) in normal mice and in alloxan-induced diabetic mice. In DPPH radical scavenging assay the IC50 value of the extract was found to be greater than 1000 μg/ml (ascorbic acid, IC50 = 14.45 μg/ml) while it was 269 μg/ml in nitric oxide (NO) scavenging assay (quercetin IC50 = 15.24 μg/ml). The amount of total flavonoid was 25 .46 mg/g and total antioxidant activity was 79.48 mg/g equivalent to Quercetin and Ascorbic acid respectively. A significant effect (p<0.05) on oral glucose tolerance was noted at the dose of 200 mg/kg and 400 mg/kg body weight in mice. At the same doses, the extract showed significant (p<0.05) reduction of blood glucose level in Alloxan-induced diabetic mice compared to the standard drug Metformin. Overall, the results of the present study indicate that the methanolic extract of Glinus oppositifolius leaves possess moderate antioxidant activity and significant antihyperglycemic activity.

Original languageEnglish (US)
Pages (from-to)50-53
Number of pages4
JournalJournal of Applied Pharmaceutical Science
Volume1
Issue number7
StatePublished - Sep 1 2011

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Molluginaceae
Hypoglycemic Agents
Antioxidants
Inhibitory Concentration 50
Alloxan
Quercetin
Glucose Tolerance Test
Flavonoids
Ascorbic Acid
Nitric Oxide
Metformin
Blood Glucose
Body Weight
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Antioxidant and antihyperglycemic activities of methanolic extract of Glinus oppositifolius leaves. / Hoque, Nazia; Imam, Mohammad Zafar; Akter, Saleha; Ahmed, Jamiuddin; Mazumder, Ehsanul Hoque; Hasan, S M Raquibul; Rana, Sohel.

In: Journal of Applied Pharmaceutical Science, Vol. 1, No. 7, 01.09.2011, p. 50-53.

Research output: Contribution to journalArticle

Hoque, Nazia ; Imam, Mohammad Zafar ; Akter, Saleha ; Ahmed, Jamiuddin ; Mazumder, Ehsanul Hoque ; Hasan, S M Raquibul ; Rana, Sohel. / Antioxidant and antihyperglycemic activities of methanolic extract of Glinus oppositifolius leaves. In: Journal of Applied Pharmaceutical Science. 2011 ; Vol. 1, No. 7. pp. 50-53.
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