Apoptosis induction in prostate cancer cells by a novel gene product, pHyde, involves caspase-3

X. Zhang, M. S. Steiner, A. Rinaldy, Yi Lu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A novel gene, pHyde, was recently cloned from Dunning rat prostate cancer cells. A recombinant adenovirus containing pHyde cDNA gene (AdpHyde) was generated to investigate the biological function of pHyde protein. AdpHyde inhibited the growth of human prostate cancer cells. Apoptosis was induced in AdpHyde transduced cells as demonstrated by DAPI (4′, 6-diamino-2-phenylindole), TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling) staining, and flow cytometry assays. Apoptosis was also induced in human xenograft prostate cancer tumors growing in nude mice following treatment with AdpHyde. AdpHyde transduction resulted in a dose-dependent stimulation of caspase-3 activity in DU145 cells which was blocked by DEVD (succinyl-Asp-Glu-Val-Asp-aldehyde) and VAD (benzyloxycarbonyl - Val - Ala - Asp -fluoromethylketone), inhibitors specifically against caspase-3. Moreover, cancer cells that lacked expression of endogenous caspase-3 were not or barely inhibited by pHyde. These results taken together suggest that pHyde inhibits cancer growth by inducing apoptosis through a caspase-3 dependent pathway.

Original languageEnglish (US)
Pages (from-to)5982-5990
Number of pages9
JournalOncogene
Volume20
Issue number42
DOIs
StatePublished - Sep 20 2001

Fingerprint

Caspase 3
Prostatic Neoplasms
Apoptosis
Genes
Neoplasms
DNA Nucleotidylexotransferase
In Situ Nick-End Labeling
Growth
Heterografts
Adenoviridae
Nude Mice
Aldehydes
Flow Cytometry
Complementary DNA
Staining and Labeling
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Apoptosis induction in prostate cancer cells by a novel gene product, pHyde, involves caspase-3. / Zhang, X.; Steiner, M. S.; Rinaldy, A.; Lu, Yi.

In: Oncogene, Vol. 20, No. 42, 20.09.2001, p. 5982-5990.

Research output: Contribution to journalArticle

Zhang, X. ; Steiner, M. S. ; Rinaldy, A. ; Lu, Yi. / Apoptosis induction in prostate cancer cells by a novel gene product, pHyde, involves caspase-3. In: Oncogene. 2001 ; Vol. 20, No. 42. pp. 5982-5990.
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