Application of field-modulated birefringence and light scattering to biosensing

Louis H. Strong, Daniel B. Hall, Clark M. Edson, Hiep Hoa Nguyen, Michael Whitt, Gyula Varadi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Superparamagnetic nanoparticles (NPs) coated with surface ligands are shown to be an effective means to impart magnetic field modulation to optical signals from targeted receptor complexes. The modulated signals they produce can be used for a number of important high throughput applications in bio-sensing including: detecting (weaponized) viruses, screening recombinant libraries of proteins, identifying pathogenic conversions of microbes, and monitoring gene amplification. We compare the results of two dynamic methods of measuring target binding to NPs: birefringence and field modulated light scattering (FMLS). These measurements reflect complementary manifestations of NP alignment (orientation) and de-alignment (relaxation) dynamics. Birefringence originates from the specific crystalline properties of a small subset of paramagnetic NPs (for example, maghemite) when oriented in a magnetic field. Upon quenching the field, it decays at a rate exhibiting the Debye-Stokes-Einstein rotational relaxation constant of target-NP complexes. Birefringence relaxation reflects the particle dynamics of the mixed suspension of NPs, with signal components weighted in proportion to the free and complexed NP size distributions. FMLS relaxation signals, on the other hand, originate predominately from the inherent optical anisotropy of the target complexes, show little contribution from non-complexed NPs when the targets are more optically anisotropic than the NPs, and provide a more direct and accurate method for determining target receptor concentrations. Several illustrations of the broad range of applications possible using these dynamic measurements and the kind of information to be derived from each detection modality will be discussed.

Original languageEnglish (US)
Title of host publicationFrontiers in Biological Detection
Subtitle of host publicationFrom Nanosensors to Systems III
DOIs
StatePublished - Apr 4 2011
EventFrontiers in Biological Detection: From Nanosensors to Systems III - San Francisco, CA, United States
Duration: Jan 22 2011Jan 23 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7888
ISSN (Print)1605-7422

Other

OtherFrontiers in Biological Detection: From Nanosensors to Systems III
CountryUnited States
CitySan Francisco, CA
Period1/22/111/23/11

Fingerprint

Birefringence
Light scattering
Nanoparticles
birefringence
light scattering
Light
nanoparticles
scattering
Magnetic Fields
alignment
Magnetic fields
Optical anisotropy
Gene Amplification
Anisotropy
viruses
microorganisms
Viruses
Recombinant Proteins
magnetic fields
genes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Strong, L. H., Hall, D. B., Edson, C. M., Nguyen, H. H., Whitt, M., & Varadi, G. (2011). Application of field-modulated birefringence and light scattering to biosensing. In Frontiers in Biological Detection: From Nanosensors to Systems III [78880P] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7888). https://doi.org/10.1117/12.875342

Application of field-modulated birefringence and light scattering to biosensing. / Strong, Louis H.; Hall, Daniel B.; Edson, Clark M.; Nguyen, Hiep Hoa; Whitt, Michael; Varadi, Gyula.

Frontiers in Biological Detection: From Nanosensors to Systems III. 2011. 78880P (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7888).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Strong, LH, Hall, DB, Edson, CM, Nguyen, HH, Whitt, M & Varadi, G 2011, Application of field-modulated birefringence and light scattering to biosensing. in Frontiers in Biological Detection: From Nanosensors to Systems III., 78880P, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7888, Frontiers in Biological Detection: From Nanosensors to Systems III, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.875342
Strong LH, Hall DB, Edson CM, Nguyen HH, Whitt M, Varadi G. Application of field-modulated birefringence and light scattering to biosensing. In Frontiers in Biological Detection: From Nanosensors to Systems III. 2011. 78880P. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.875342
Strong, Louis H. ; Hall, Daniel B. ; Edson, Clark M. ; Nguyen, Hiep Hoa ; Whitt, Michael ; Varadi, Gyula. / Application of field-modulated birefringence and light scattering to biosensing. Frontiers in Biological Detection: From Nanosensors to Systems III. 2011. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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