Application of optical imaging to study of extrapulmonary spread by tuberculosis

Ying Kong, Selvakumar Subbian, Suat L G Cirillo, Jeffrey D. Cirillo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The incidence of extrapulmonary tuberculosis is increasing, possibly due to the high frequency of co-infection with HIV. Extrapulmonary infections complicate diagnosis, have higher mortality rates and are more difficult to treat. Insight into the mechanisms involved in extrapulmonary spread of tuberculosis is critical to improving management. We set out to better understand extrapulmonary spread kinetics in mice and guinea pigs as well as the effects of infectious dose. We found that extrapulmonary spread occurs at a discrete time point when infected by low-dose aerosol, but at high-dose aerosol it occurs within the first 24h. The ability to follow tuberculosis in real-time during infection would allow us to better address the mechanisms involved. We found that mycobacteria can be optically imaged after pulmonary infection in the mouse lung, suggesting that this technology could be applied to study of extrapulmonary spread of tuberculosis.

Original languageEnglish (US)
JournalTuberculosis
Volume89
Issue numberSUPPL.1
DOIs
StatePublished - Dec 1 2009

Fingerprint

Optical Imaging
Tuberculosis
Aerosols
Infection
Lung
Mycobacterium
Coinfection
Guinea Pigs
HIV
Technology
Mortality
Incidence

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Application of optical imaging to study of extrapulmonary spread by tuberculosis. / Kong, Ying; Subbian, Selvakumar; Cirillo, Suat L G; Cirillo, Jeffrey D.

In: Tuberculosis, Vol. 89, No. SUPPL.1, 01.12.2009.

Research output: Contribution to journalArticle

Kong, Ying ; Subbian, Selvakumar ; Cirillo, Suat L G ; Cirillo, Jeffrey D. / Application of optical imaging to study of extrapulmonary spread by tuberculosis. In: Tuberculosis. 2009 ; Vol. 89, No. SUPPL.1.
@article{f3dafdc0105f43c2aa8199a2f58f363d,
title = "Application of optical imaging to study of extrapulmonary spread by tuberculosis",
abstract = "The incidence of extrapulmonary tuberculosis is increasing, possibly due to the high frequency of co-infection with HIV. Extrapulmonary infections complicate diagnosis, have higher mortality rates and are more difficult to treat. Insight into the mechanisms involved in extrapulmonary spread of tuberculosis is critical to improving management. We set out to better understand extrapulmonary spread kinetics in mice and guinea pigs as well as the effects of infectious dose. We found that extrapulmonary spread occurs at a discrete time point when infected by low-dose aerosol, but at high-dose aerosol it occurs within the first 24h. The ability to follow tuberculosis in real-time during infection would allow us to better address the mechanisms involved. We found that mycobacteria can be optically imaged after pulmonary infection in the mouse lung, suggesting that this technology could be applied to study of extrapulmonary spread of tuberculosis.",
author = "Ying Kong and Selvakumar Subbian and Cirillo, {Suat L G} and Cirillo, {Jeffrey D.}",
year = "2009",
month = "12",
day = "1",
doi = "10.1016/S1472-9792(09)70006-X",
language = "English (US)",
volume = "89",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",
number = "SUPPL.1",

}

TY - JOUR

T1 - Application of optical imaging to study of extrapulmonary spread by tuberculosis

AU - Kong, Ying

AU - Subbian, Selvakumar

AU - Cirillo, Suat L G

AU - Cirillo, Jeffrey D.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - The incidence of extrapulmonary tuberculosis is increasing, possibly due to the high frequency of co-infection with HIV. Extrapulmonary infections complicate diagnosis, have higher mortality rates and are more difficult to treat. Insight into the mechanisms involved in extrapulmonary spread of tuberculosis is critical to improving management. We set out to better understand extrapulmonary spread kinetics in mice and guinea pigs as well as the effects of infectious dose. We found that extrapulmonary spread occurs at a discrete time point when infected by low-dose aerosol, but at high-dose aerosol it occurs within the first 24h. The ability to follow tuberculosis in real-time during infection would allow us to better address the mechanisms involved. We found that mycobacteria can be optically imaged after pulmonary infection in the mouse lung, suggesting that this technology could be applied to study of extrapulmonary spread of tuberculosis.

AB - The incidence of extrapulmonary tuberculosis is increasing, possibly due to the high frequency of co-infection with HIV. Extrapulmonary infections complicate diagnosis, have higher mortality rates and are more difficult to treat. Insight into the mechanisms involved in extrapulmonary spread of tuberculosis is critical to improving management. We set out to better understand extrapulmonary spread kinetics in mice and guinea pigs as well as the effects of infectious dose. We found that extrapulmonary spread occurs at a discrete time point when infected by low-dose aerosol, but at high-dose aerosol it occurs within the first 24h. The ability to follow tuberculosis in real-time during infection would allow us to better address the mechanisms involved. We found that mycobacteria can be optically imaged after pulmonary infection in the mouse lung, suggesting that this technology could be applied to study of extrapulmonary spread of tuberculosis.

UR - http://www.scopus.com/inward/record.url?scp=72049085779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72049085779&partnerID=8YFLogxK

U2 - 10.1016/S1472-9792(09)70006-X

DO - 10.1016/S1472-9792(09)70006-X

M3 - Article

VL - 89

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

IS - SUPPL.1

ER -