Aqueous aldehyde (FAGLU) histofluorescence for catecholamines in 2 μm sections using polyethylene glycol embedding

Jan Schöler, William Armstrong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A technique is described which couples the aqueous aldehyde fluorescence (Faglu) method [5,6] with polyethylene glycol (PEG) embedding to obtain sections of brain as thin as 2 μm for light microscopic examination. Blocks from rat brains perfused with Faglu solution were embedded in PEG, sectioned on a rotary microtome and examined for amine fluorescence. Both catecholaminergic perikarya and their terminal arborizations were observed with high resolution in 2 μm sections. Single neurons could be traced in serial sections and varicosities as well as intervaricose segments were visible in terminal regions. This technique offers the further advantage of fixation compatible with electron microscopy, immunohistochemistry and other neuroanatomical methods. PEG-embedding of Faglu-fixed tissue thus provides a useful addition to existing fluorescence histochemical techniques for the visualization of catecholamines.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalBrain Research Bulletin
Volume9
Issue number1-6
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

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Aldehydes
Catecholamines
Fluorescence
Brain
Amines
Electron Microscopy
Immunohistochemistry
Neurons
Light

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Aqueous aldehyde (FAGLU) histofluorescence for catecholamines in 2 μm sections using polyethylene glycol embedding. / Schöler, Jan; Armstrong, William.

In: Brain Research Bulletin, Vol. 9, No. 1-6, 01.01.1982, p. 27-31.

Research output: Contribution to journalArticle

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