Aqueous extract of walnut (Juglans regia L.) protects mice against cyclophosphamide-induced biochemical toxicity

R. Haque, Bilal Hafeez, S. Parvez, S. Pandey, I. Sayeed, M. Ali, S. Raisuddin

Research output: Contribution to journalArticle

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Abstract

Walnut (Juglans regia L.) is extensively used in traditional systems of medicine for treatment of various ailments. It is described as an anticancer, tonic, blood purifier and detoxifier agent. The present study was undertaken to investigate modulatory effects of walnut extract on the toxicity of an anticancer drug, cyclophosphamide (CP) with special reference to protection against disruption of drug metabolizing and antioxidant enzymes. Plant extract+CP group animals showed restoration in the level of cytochrome P450 (CYP) content and in the activities of glutathione S-transferase (GST), glutathione peroxidase (GP) and catalase (CAT) in both liver and kidneys. But plant extract restored the activity of super-oxide dismutase (SOD) and the level of reduced glutathione (GSH) in the kidneys only when compared with CP-treated animals. Plant extract treatment alone caused significant reduction in the content of CYP in the kidneys mainly. The extract showed a significant increase in the level of GSH and in the activities of GP in both the tissues and CAT in liver only, whereas no significant change was observed in the activities of GST and SOD. CP treatment resulted in a significant (P < 0.01) increase in the lipid peroxidation (LPO) in the liver and kidneys compared with controls, while the extract+CP treated group showed a significant decrease in the LPO in liver and kidneys when compared with the CP-treated group. The study shows that the use of J. regia extract might be helpful in abrogation of CP toxicity during the chemotherapy.

Original languageEnglish (US)
Pages (from-to)473-480
Number of pages8
JournalHuman and Experimental Toxicology
Volume22
Issue number9
DOIs
StatePublished - Sep 1 2003

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Juglans
Cyclophosphamide
Toxicity
Liver
Plant Extracts
Kidney
Glutathione Peroxidase
Glutathione Transferase
Catalase
Cytochrome P-450 Enzyme System
Oxides
Lipid Peroxidation
Animals
Lipids
Chemotherapy
Traditional Medicine
Systems Analysis
Drug-Related Side Effects and Adverse Reactions
Pharmaceutical Preparations
Restoration

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Aqueous extract of walnut (Juglans regia L.) protects mice against cyclophosphamide-induced biochemical toxicity. / Haque, R.; Hafeez, Bilal; Parvez, S.; Pandey, S.; Sayeed, I.; Ali, M.; Raisuddin, S.

In: Human and Experimental Toxicology, Vol. 22, No. 9, 01.09.2003, p. 473-480.

Research output: Contribution to journalArticle

Haque, R. ; Hafeez, Bilal ; Parvez, S. ; Pandey, S. ; Sayeed, I. ; Ali, M. ; Raisuddin, S. / Aqueous extract of walnut (Juglans regia L.) protects mice against cyclophosphamide-induced biochemical toxicity. In: Human and Experimental Toxicology. 2003 ; Vol. 22, No. 9. pp. 473-480.
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