Arachidonic acid activation of translation initiation signaling in vascular smooth muscle cells

Indira Neeli, Chandrahasa R. Yellaturu, Rao Gadiparthi

Research output: Contribution to journalArticle

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Abstract

To understand the role of arachidonic acid (AA) in regulating vascular smooth muscle cell (VSMC) growth, its effects on phosphorylation of Akt, S6K1, ribosomal protein S6, 4EBP1, and eIF4E were studied. Arachidonic acid stimulated phosphorylation of Akt, S6K1, ribosomal protein S6, 4EBP1, and eIF4E in a time-dependent manner in VSMC. Arachidonic acid stimulation of phosphorylation of the above signaling molecules is specific, as these events were not affected by other unsaturated or saturated fatty acids. Metabolic conversion of AA via the LOX/MOX and/or COX pathways, to some extent, was required for its effects on the phosphorylation of Akt, S6K1, ribosomal protein S6, 4EBP1, and eIF4E. In addition, AA increased PI3K activity in a time-dependent manner in VSMC. LY294002, an inhibitor of PI3K, completely blocked AA-induced phosphorylation of Akt, S6K1, ribosomal protein S6, 4EBP1, and eIF4E, suggesting a role for PI3K in these effects. Consistent with its effects on translation initiation signaling events, AA induced global protein synthesis in VSMC and this response was dependent, to some extent, on its metabolism via the LOX/MOX and/or COX pathways, and mediated by the PI3K/Akt/mTOR pathway. Thus, the above observations provide the first biochemical evidence for the role of AA in the activation of translation initiation signaling in VSMC.

Original languageEnglish (US)
Pages (from-to)755-761
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume309
Issue number4
DOIs
StatePublished - Oct 3 2003

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Vascular Smooth Muscle
Arachidonic Acid
Smooth Muscle Myocytes
Muscle
Phosphorylation
Chemical activation
Ribosomal Protein S6
Cells
Phosphatidylinositol 3-Kinases
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Cell growth
Unsaturated Fatty Acids
Metabolism
Fatty Acids
Molecules
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Arachidonic acid activation of translation initiation signaling in vascular smooth muscle cells. / Neeli, Indira; Yellaturu, Chandrahasa R.; Gadiparthi, Rao.

In: Biochemical and Biophysical Research Communications, Vol. 309, No. 4, 03.10.2003, p. 755-761.

Research output: Contribution to journalArticle

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