Arrest and extravasation of neoplastic cells - An electron microscopy study of serial sections at sequential stages

Emilio A. Machado, David Gerard, James R. Mitchell, Bismarck B. Lozzio, Carmen B. Lozzio

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The morphological aspects of the arrest and extravasation of malignant cells of human origin (K-562 cell line) in the lungs of athymic (nude) and asplenic-athymic (lasat) mice were studied by electron microscopy examination of serial sections. The specimens were obtained at sequential stages after the sc inoculation into newborn mice of 107 malignant cells. K-562 cells (105) were also injected iv into control groups of nude and lasat mice to assess the influence of the route of inoculation on the in vivo behavior of K-562 cells. Our results demonstrated that K-562 cells were arrested and proliferated within the pulmonary capillaries without the participation of platelets or fibrin. The neoplastic cells extravasated by attrition and penetration of the endothelium (rather than by diapedesis) and continued to proliferate in the interstitial tissue of the lung, developing into neoplastic nodules. Following iv injection, K-562 cells induced the formation of platelet-tumor cell aggregates within the pulmonary capillaries. However, under these conditions, the neoplastic cells did not adhere to the endothelium nor did they proliferate or extravasate. These aggregates were flushed out by the circulation, restoring the permeability of the capillaries.

Original languageEnglish (US)
Pages (from-to)73-89
Number of pages17
JournalVirchows Archiv A Pathological Anatomy and Histology
Volume396
Issue number1
DOIs
StatePublished - Jun 1 1982

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Electron Microscopy
Lung
Nude Mice
Endothelium
Blood Platelets
Transendothelial and Transepithelial Migration
Capillary Permeability
Fibrin
Cell Line
Control Groups
Injections
Neoplasms

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Pathology and Forensic Medicine
  • Histology

Cite this

Arrest and extravasation of neoplastic cells - An electron microscopy study of serial sections at sequential stages. / Machado, Emilio A.; Gerard, David; Mitchell, James R.; Lozzio, Bismarck B.; Lozzio, Carmen B.

In: Virchows Archiv A Pathological Anatomy and Histology, Vol. 396, No. 1, 01.06.1982, p. 73-89.

Research output: Contribution to journalArticle

Machado, Emilio A. ; Gerard, David ; Mitchell, James R. ; Lozzio, Bismarck B. ; Lozzio, Carmen B. / Arrest and extravasation of neoplastic cells - An electron microscopy study of serial sections at sequential stages. In: Virchows Archiv A Pathological Anatomy and Histology. 1982 ; Vol. 396, No. 1. pp. 73-89.
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