Ascorbic acid attenuates hyperoxia-compromised host defense against pulmonary bacterial infection

Vivek Patel, Vaishali Sampat, Michael Graham Espey, Ravikumar Sitapara, Haichao Wang, Xiaojing Yang, Charles R. Ashby, Douglas D. Thomas, Lin L. Mantell

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Supraphysiological concentrations of oxygen (hyperoxia) can compromise host defense and increase susceptibility to bacterial infections, causing ventilator-associated pneumonia. The phagocytic activity of macrophages is impaired by hyperoxia-induced increases in the levels of reactive oxygen species (ROS) and extracellular highmobility group box protein B1 (HMGB1). Ascorbic acid (AA), an essential nutrient and antioxidant, has been shown to be beneficial in various animal models of ROS-mediated diseases. The aim of this study was to determine whether AA could attenuate hyperoxiacompromised hostdefense and improvemacrophage functions against bacterial infections. C57BL/6 male mice were exposed to hyperoxia (≥98% O2, 48 h), followed by intratracheal inoculation with Pseudomonas aeruginosa, and simultaneous intraperitoneal administration of AA. AA(50mg/kg) significantly improved bacterial clearance in the lungs and airways, and significantly reduced HMGB1 accumulation in the airways. The incubation of RAW264.7 cells (a macrophage-like cell line) with AA (0-1,000 μM) before hyperoxic exposure (95%O2) stabilized the phagocytic activity ofmacrophages in a concentration-dependent manner. The AA-enhanced macrophage function was associated with significantly decreased production of intracellular ROS and accumulation of extracellular HMGB1. These data suggest that AA supplementation can prevent or attenuate the development of ventilator-associated pneumonia in patients receiving oxygen support.

Original languageEnglish (US)
Pages (from-to)511-520
Number of pages10
JournalAmerican journal of respiratory cell and molecular biology
Volume55
Issue number4
DOIs
StatePublished - Oct 1 2016

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Hyperoxia
Bacterial Infections
Ascorbic Acid
Lung
Macrophages
Ventilator-Associated Pneumonia
Reactive Oxygen Species
Bridge clearances
Oxygen
Proteins
Pseudomonas aeruginosa
Nutrients
Animals
Animal Models
Antioxidants
Cells
Cell Line
Food

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Ascorbic acid attenuates hyperoxia-compromised host defense against pulmonary bacterial infection. / Patel, Vivek; Sampat, Vaishali; Espey, Michael Graham; Sitapara, Ravikumar; Wang, Haichao; Yang, Xiaojing; Ashby, Charles R.; Thomas, Douglas D.; Mantell, Lin L.

In: American journal of respiratory cell and molecular biology, Vol. 55, No. 4, 01.10.2016, p. 511-520.

Research output: Contribution to journalArticle

Patel, V, Sampat, V, Espey, MG, Sitapara, R, Wang, H, Yang, X, Ashby, CR, Thomas, DD & Mantell, LL 2016, 'Ascorbic acid attenuates hyperoxia-compromised host defense against pulmonary bacterial infection', American journal of respiratory cell and molecular biology, vol. 55, no. 4, pp. 511-520. https://doi.org/10.1165/rcmb.2015-0310OC
Patel, Vivek ; Sampat, Vaishali ; Espey, Michael Graham ; Sitapara, Ravikumar ; Wang, Haichao ; Yang, Xiaojing ; Ashby, Charles R. ; Thomas, Douglas D. ; Mantell, Lin L. / Ascorbic acid attenuates hyperoxia-compromised host defense against pulmonary bacterial infection. In: American journal of respiratory cell and molecular biology. 2016 ; Vol. 55, No. 4. pp. 511-520.
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