Aspergillus fumigatus calcipressin CbpA is involved in hyphal growth and calcium homeostasis

Nadthanan Pinchai, B. Zachary Perfect, Praveen Rao Juvvadi, Jarrod Fortwendel, Robert A. Cramer, Yohannes G. Asfaw, Joseph Heitman, John R. Perfect, William J. Steinbach

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Calcineurin is a conserved protein phosphatase that plays a critical role in Ca 2+ signaling and stress responses. Previously, a new class of conserved calcineurin-binding proteins, the calcipressins, was identified. However, the role of these proteins remains controversial, and both inhibitory and stimulatory effects on calcineurin were observed. In this study, we investigate the role of CbpA, the Aspergillus fumigatus member of the calcipressin family, and report that deletion of the cbpA gene resulted in reduced hyphal growth and limited attenuated virulence. Interestingly, under high-calcium-level conditions, the ΔcbpA strain displayed improved Ca 2+ tolerance compared to the wild-type strain and revealed increased expression of vcxA, chsA, and cnaA, which encode the vacuolar Ca 2+/H + exchanger VcxA, chitin synthase A, and the calcineurin catalytic subunit CnaA, respectively. The increased transcript levels of these three genes were reversed in the presence of the calcineurin inhibitor FK506, indicating a calcineurin-dependent mechanism. Overexpression of cbpA resulted in decreased transcription of vcxA, chsA, and cnaA, associated with wild-type sensitivity to Ca 2+. Taken together, our study highlights the importance of CbpA in the regulation of hyphal growth and calcium adaptation of A. fumigatus and provides evidence that CbpA may serve as a feedback inhibitor in some aspects of calcineurin functions.

Original languageEnglish (US)
Pages (from-to)511-519
Number of pages9
JournalEukaryotic Cell
Volume8
Issue number4
DOIs
StatePublished - Apr 1 2009

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Aspergillus fumigatus
Calcineurin
Homeostasis
Calcium
Growth
Chitin Synthase
Phosphoprotein Phosphatases
Gene Deletion
Tacrolimus
Virulence
Catalytic Domain
Carrier Proteins
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Pinchai, N., Perfect, B. Z., Juvvadi, P. R., Fortwendel, J., Cramer, R. A., Asfaw, Y. G., ... Steinbach, W. J. (2009). Aspergillus fumigatus calcipressin CbpA is involved in hyphal growth and calcium homeostasis. Eukaryotic Cell, 8(4), 511-519. https://doi.org/10.1128/EC.00336-08

Aspergillus fumigatus calcipressin CbpA is involved in hyphal growth and calcium homeostasis. / Pinchai, Nadthanan; Perfect, B. Zachary; Juvvadi, Praveen Rao; Fortwendel, Jarrod; Cramer, Robert A.; Asfaw, Yohannes G.; Heitman, Joseph; Perfect, John R.; Steinbach, William J.

In: Eukaryotic Cell, Vol. 8, No. 4, 01.04.2009, p. 511-519.

Research output: Contribution to journalArticle

Pinchai, N, Perfect, BZ, Juvvadi, PR, Fortwendel, J, Cramer, RA, Asfaw, YG, Heitman, J, Perfect, JR & Steinbach, WJ 2009, 'Aspergillus fumigatus calcipressin CbpA is involved in hyphal growth and calcium homeostasis', Eukaryotic Cell, vol. 8, no. 4, pp. 511-519. https://doi.org/10.1128/EC.00336-08
Pinchai, Nadthanan ; Perfect, B. Zachary ; Juvvadi, Praveen Rao ; Fortwendel, Jarrod ; Cramer, Robert A. ; Asfaw, Yohannes G. ; Heitman, Joseph ; Perfect, John R. ; Steinbach, William J. / Aspergillus fumigatus calcipressin CbpA is involved in hyphal growth and calcium homeostasis. In: Eukaryotic Cell. 2009 ; Vol. 8, No. 4. pp. 511-519.
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