Assay and recommendations for the detection of vapour-phase-mediated antimicrobial activities

Adam F. Feyaerts, Lotte Mathé, Walter Luyten, Helene Tournu, Katrien Van Dyck, Lize Broekx, Patrick Van Dijck

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antimicrobial activity assays can be carried out in aqueous solutions using multi-well plates. However, some bioactive compounds are volatile and can cause effects at a distance. To detect such vapour-phase-mediated antimicrobial activity, we introduce the vapour-phase-mediated patch assay, a simple bioassay that uses standard microtitre plates. As a proof-of-principle, we use the vapour-phase-mediated patch assay to test a small but chemically diverse set of selected essential oils with known antifungal activities i.e. Origanum compactum, Artemisia dracunculus, Cinnamomum camphora ct linalool, Cinnamomum cassia and Melissa officinalis, as well as their corresponding major components carvacrol, estragole, linalool, trans-cinnamaldehyde and citral, against two pathogenic Candida species. As all but one of the tested essential oils i.e. Artemisia dracunculus and its corresponding component estragole, showed vapour-phase-mediated antimicrobial activity, we conclude that it is a rather common characteristic of essential oils and their components, that should always be taken into consideration. Additionally, we provide suggestions to prevent false positive results due to possible vapour-phase-mediated antimicrobial activities in bioactivity tests.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalFlavour and Fragrance Journal
Volume32
Issue number5
DOIs
StatePublished - Sep 1 2017

Fingerprint

Volatile Oils
vapors
Artemisia
Assays
citral
anti-infective agents
Vapors
Artemisia dracunculus
Cinnamomum camphora
Cinnamomum aromaticum
methyl chavicol
assays
Melissa
essential oils
Origanum
linalool
Candida
Biological Assay
Melissa officinalis
Cinnamomum

All Science Journal Classification (ASJC) codes

  • Food Science
  • Chemistry(all)

Cite this

Feyaerts, A. F., Mathé, L., Luyten, W., Tournu, H., Van Dyck, K., Broekx, L., & Van Dijck, P. (2017). Assay and recommendations for the detection of vapour-phase-mediated antimicrobial activities. Flavour and Fragrance Journal, 32(5), 347-353. https://doi.org/10.1002/ffj.3400

Assay and recommendations for the detection of vapour-phase-mediated antimicrobial activities. / Feyaerts, Adam F.; Mathé, Lotte; Luyten, Walter; Tournu, Helene; Van Dyck, Katrien; Broekx, Lize; Van Dijck, Patrick.

In: Flavour and Fragrance Journal, Vol. 32, No. 5, 01.09.2017, p. 347-353.

Research output: Contribution to journalArticle

Feyaerts, AF, Mathé, L, Luyten, W, Tournu, H, Van Dyck, K, Broekx, L & Van Dijck, P 2017, 'Assay and recommendations for the detection of vapour-phase-mediated antimicrobial activities', Flavour and Fragrance Journal, vol. 32, no. 5, pp. 347-353. https://doi.org/10.1002/ffj.3400
Feyaerts, Adam F. ; Mathé, Lotte ; Luyten, Walter ; Tournu, Helene ; Van Dyck, Katrien ; Broekx, Lize ; Van Dijck, Patrick. / Assay and recommendations for the detection of vapour-phase-mediated antimicrobial activities. In: Flavour and Fragrance Journal. 2017 ; Vol. 32, No. 5. pp. 347-353.
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