Assessment of bioactive and bio-adhesive therapies to enhance stem cell attachment to root surface dentine

M. A. Elseed, P. E. Murray, Franklin Garcia-Godoy, K. N. Namerow

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Elseed MA, Murray PE, Garcia-Godoy F, Namerow KN. Assessment of bioactive and bio-adhesive therapies to enhance stem cell attachment to root surface dentine. International Endodontic Journal, 42, 576-583, 2009.AbstractAim To compare bioactive and bio-adhesive therapies to enhance stem cell attachment to the root dentine of human teeth. Methodology Dentine slabs (n = 72) were cut from the lower 3 mm of the roots of extracted human permanent teeth. The root dentine slabs were untreated, or coated with bio-adhesive, or human recombinant transforming growth factor-beta1 (hrTGF-B1), or human recombinant bone morphogenic protein-2 (hrBMP-2). The dentine slabs were placed with the root surface in contact with confluent periodontal stem cell (PSC) cultures using aseptic techniques. The cells and dentine slabs were submerged in culture media for 4, 24 and 72 h. The specimens were fixed in formalin, dehydrated and processed for scanning electron microscopy (SEM). Results SEM micrographs at ×2000 magnification revealed PSC extensive adherence to root dentine for all of the bio-adhesive and bioactive treatments. The addition of bioactive molecules did not improve PSC attachment. Few cells attached to the negative control treatments. Conclusions Bio-adhesive and bioactive growth factors were not needed to promote PSC attachment to the root dentine of human teeth, because it already appears to have good natural properties to promote PSC attachment. This suggests PSC can be used for the clinical replantation of avulsed teeth without the need for bio-adhesive and bioactive treatments.

Original languageEnglish (US)
Pages (from-to)576-583
Number of pages8
JournalInternational Endodontic Journal
Volume42
Issue number7
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

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Dentin
Adhesives
Stem Cells
Tooth
Therapeutics
Electron Scanning Microscopy
Tooth Avulsion
Transforming Growth Factor beta1
Replantation
Endodontics
Formaldehyde
Culture Media
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Assessment of bioactive and bio-adhesive therapies to enhance stem cell attachment to root surface dentine. / Elseed, M. A.; Murray, P. E.; Garcia-Godoy, Franklin; Namerow, K. N.

In: International Endodontic Journal, Vol. 42, No. 7, 01.07.2009, p. 576-583.

Research output: Contribution to journalArticle

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abstract = "Elseed MA, Murray PE, Garcia-Godoy F, Namerow KN. Assessment of bioactive and bio-adhesive therapies to enhance stem cell attachment to root surface dentine. International Endodontic Journal, 42, 576-583, 2009.AbstractAim To compare bioactive and bio-adhesive therapies to enhance stem cell attachment to the root dentine of human teeth. Methodology Dentine slabs (n = 72) were cut from the lower 3 mm of the roots of extracted human permanent teeth. The root dentine slabs were untreated, or coated with bio-adhesive, or human recombinant transforming growth factor-beta1 (hrTGF-B1), or human recombinant bone morphogenic protein-2 (hrBMP-2). The dentine slabs were placed with the root surface in contact with confluent periodontal stem cell (PSC) cultures using aseptic techniques. The cells and dentine slabs were submerged in culture media for 4, 24 and 72 h. The specimens were fixed in formalin, dehydrated and processed for scanning electron microscopy (SEM). Results SEM micrographs at ×2000 magnification revealed PSC extensive adherence to root dentine for all of the bio-adhesive and bioactive treatments. The addition of bioactive molecules did not improve PSC attachment. Few cells attached to the negative control treatments. Conclusions Bio-adhesive and bioactive growth factors were not needed to promote PSC attachment to the root dentine of human teeth, because it already appears to have good natural properties to promote PSC attachment. This suggests PSC can be used for the clinical replantation of avulsed teeth without the need for bio-adhesive and bioactive treatments.",
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N2 - Elseed MA, Murray PE, Garcia-Godoy F, Namerow KN. Assessment of bioactive and bio-adhesive therapies to enhance stem cell attachment to root surface dentine. International Endodontic Journal, 42, 576-583, 2009.AbstractAim To compare bioactive and bio-adhesive therapies to enhance stem cell attachment to the root dentine of human teeth. Methodology Dentine slabs (n = 72) were cut from the lower 3 mm of the roots of extracted human permanent teeth. The root dentine slabs were untreated, or coated with bio-adhesive, or human recombinant transforming growth factor-beta1 (hrTGF-B1), or human recombinant bone morphogenic protein-2 (hrBMP-2). The dentine slabs were placed with the root surface in contact with confluent periodontal stem cell (PSC) cultures using aseptic techniques. The cells and dentine slabs were submerged in culture media for 4, 24 and 72 h. The specimens were fixed in formalin, dehydrated and processed for scanning electron microscopy (SEM). Results SEM micrographs at ×2000 magnification revealed PSC extensive adherence to root dentine for all of the bio-adhesive and bioactive treatments. The addition of bioactive molecules did not improve PSC attachment. Few cells attached to the negative control treatments. Conclusions Bio-adhesive and bioactive growth factors were not needed to promote PSC attachment to the root dentine of human teeth, because it already appears to have good natural properties to promote PSC attachment. This suggests PSC can be used for the clinical replantation of avulsed teeth without the need for bio-adhesive and bioactive treatments.

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