Assessment of counting efficiencies for labeled protein and other macromolecules in filter disk assays

Elizabeth B. Albright, Thaddeus Nowak, Hamish N. Munro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A several-fold greater counting efficiency is observed for protein labeled with [3H]leucine than for free [3H]leucine using a conventional filter disk assay. A similar, though less marked, effect is noted for 14C-labeled molecules. These results are comparable to those reported by others for counting efficiencies of labeled DNA and deoxynucleotides and illustrate the generality of this effect with regard to macromolecules and their low-molecular weight precursors. This phenomenon, presumably due to differences in the distribution of large and small molecules within filters, gives rise to errors in the quantitation of macromolecule synthesis if a counting efficiency identical to that of the precursor is assumed to apply. A convenient method for determining counting efficiencies of various molecules bound to filters is presented which eliminates this problem.

Original languageEnglish (US)
Pages (from-to)258-263
Number of pages6
JournalAnalytical Biochemistry
Volume91
Issue number1
DOIs
StatePublished - Jan 1 1978

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Macromolecules
Leucine
Assays
Molecules
Proteins
Molecular Weight
DNA
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Assessment of counting efficiencies for labeled protein and other macromolecules in filter disk assays. / Albright, Elizabeth B.; Nowak, Thaddeus; Munro, Hamish N.

In: Analytical Biochemistry, Vol. 91, No. 1, 01.01.1978, p. 258-263.

Research output: Contribution to journalArticle

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