Assignment of the gene for adrenal p450cl7 (steroid 17α-hydr0xylase/17,20 lyase) to human chromosome 10

Karla Matteson, James Picado-Leonard, Bon Chu Chung, T. K. Mohandas, Walter L. Miller

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

P450cl7 is the single enzyme mediating both 17α-hydroxylase and 17,20 lyase activities. We identified several human P450cl7 cDNA clones in a human adrenal cDNA library we constructed in XgtlO. A short clone containing the 3’-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450cl7 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450cl7 gene lies on chromosome 10. The chromosomal locations of the other P450cl7 genes could not be determined.

Original languageEnglish (US)
Pages (from-to)789-791
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume63
Issue number3
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Steroid 17-alpha-Hydroxylase
Chromosomes, Human, Pair 10
Human Chromosomes
Chromosomes
Genes
Steroids
Hybrid Cells
Complementary DNA
Clone Cells
DNA
DNA Restriction Enzymes
Cell Line
Mixed Function Oxygenases
Gene Library
Human Genome
Southern Blotting
Cells
Enzymes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Assignment of the gene for adrenal p450cl7 (steroid 17α-hydr0xylase/17,20 lyase) to human chromosome 10. / Matteson, Karla; Picado-Leonard, James; Chung, Bon Chu; Mohandas, T. K.; Miller, Walter L.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 63, No. 3, 01.01.1986, p. 789-791.

Research output: Contribution to journalArticle

Matteson, Karla ; Picado-Leonard, James ; Chung, Bon Chu ; Mohandas, T. K. ; Miller, Walter L. / Assignment of the gene for adrenal p450cl7 (steroid 17α-hydr0xylase/17,20 lyase) to human chromosome 10. In: Journal of Clinical Endocrinology and Metabolism. 1986 ; Vol. 63, No. 3. pp. 789-791.
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