Association of the 4g/5g polymorphism of plasminogen activator inhibitor-1 gene with sudden sensorineural hearing loss. A case control study

Seong Ho Cho, Haimei Chen, Il Soo Kim, Chio Yokose, Joseph Kang, David Cho, Chun Cai, Silvia Palma, Micol Busi, Alessandro Martini, Tae J. Yoo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: The 5 G/5 G genotype of PAI-1 polymorphism is linked to decreased plasminogen activator inhibitor-1 (PAI-1) levels and it has been suggested that lower PAI-1 levels may provide protective effects on inflammation, local microcirculatory disturbance, and fibrotic changes, which are likely associated with development of sudden sensorineural hearing loss (SSNHL). Methods: The association of the 4 G/5 G PAI-1 polymorphism with the development and clinical outcome of SSNHL is evaluated via a case control study. 103 patients with SSNHL and 113 age and sex-matched controls were enrolled at University of Ferrara, Italy and hearing loss outcome was measured at least 3 months after the onset of hearing loss. DNA was isolated from peripheral blood using the QIAamp kit and the 4 G/5 G polymorphism in the -675 promoter region was genotyped with an allele-specific PCR. Genotype distribution was tested in patients and compared to controls by chi-square and odd-ratio analysis. The codominant and recessive models were used for the multiple logistic regression analyses of the PAI-1 gene allele. Results: In this population, 5 G/5 G genotype had a two-time lower frequency in SSNHL patients compared to healthy controls (15.5% vs 30.1%) and was associated with decreased odds compared to 4 G/5 G genotype (OR 0.37, 95% CI 0.19- 0.75, p = 0.005). In addition, the patients with 5 G/5 G genotype showed a trend of more than 2 times higher ratio of hearing recovery (> 20 dB) after systemic corticosteroid treatment compared to 4 G/5 G genotype (OR 2.3, 95% CI 0.32 - 16.83, p = 0.39), suggesting a better clinical outcome. Conclusions: The 5 G/5 G genotype of PAI-1may be associated with a reduced risk of SSNHL in the Italian population.

Original languageEnglish (US)
Article number5
JournalBMC Ear, Nose and Throat Disorders
Volume12
Issue number1
DOIs
StatePublished - Sep 10 2012

Fingerprint

Sudden Hearing Loss
Sensorineural Hearing Loss
Plasminogen Activator Inhibitor 1
Case-Control Studies
Genotype
Genes
Hearing Loss
Alleles
Genetic Promoter Regions
Italy
Population
Hearing
Adrenal Cortex Hormones
Logistic Models
Odds Ratio
Regression Analysis
Inflammation
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Association of the 4g/5g polymorphism of plasminogen activator inhibitor-1 gene with sudden sensorineural hearing loss. A case control study. / Cho, Seong Ho; Chen, Haimei; Kim, Il Soo; Yokose, Chio; Kang, Joseph; Cho, David; Cai, Chun; Palma, Silvia; Busi, Micol; Martini, Alessandro; Yoo, Tae J.

In: BMC Ear, Nose and Throat Disorders, Vol. 12, No. 1, 5, 10.09.2012.

Research output: Contribution to journalArticle

Cho, Seong Ho ; Chen, Haimei ; Kim, Il Soo ; Yokose, Chio ; Kang, Joseph ; Cho, David ; Cai, Chun ; Palma, Silvia ; Busi, Micol ; Martini, Alessandro ; Yoo, Tae J. / Association of the 4g/5g polymorphism of plasminogen activator inhibitor-1 gene with sudden sensorineural hearing loss. A case control study. In: BMC Ear, Nose and Throat Disorders. 2012 ; Vol. 12, No. 1.
@article{682933b1d1564db0aa5135b4a2052986,
title = "Association of the 4g/5g polymorphism of plasminogen activator inhibitor-1 gene with sudden sensorineural hearing loss. A case control study",
abstract = "Background: The 5 G/5 G genotype of PAI-1 polymorphism is linked to decreased plasminogen activator inhibitor-1 (PAI-1) levels and it has been suggested that lower PAI-1 levels may provide protective effects on inflammation, local microcirculatory disturbance, and fibrotic changes, which are likely associated with development of sudden sensorineural hearing loss (SSNHL). Methods: The association of the 4 G/5 G PAI-1 polymorphism with the development and clinical outcome of SSNHL is evaluated via a case control study. 103 patients with SSNHL and 113 age and sex-matched controls were enrolled at University of Ferrara, Italy and hearing loss outcome was measured at least 3 months after the onset of hearing loss. DNA was isolated from peripheral blood using the QIAamp kit and the 4 G/5 G polymorphism in the -675 promoter region was genotyped with an allele-specific PCR. Genotype distribution was tested in patients and compared to controls by chi-square and odd-ratio analysis. The codominant and recessive models were used for the multiple logistic regression analyses of the PAI-1 gene allele. Results: In this population, 5 G/5 G genotype had a two-time lower frequency in SSNHL patients compared to healthy controls (15.5{\%} vs 30.1{\%}) and was associated with decreased odds compared to 4 G/5 G genotype (OR 0.37, 95{\%} CI 0.19- 0.75, p = 0.005). In addition, the patients with 5 G/5 G genotype showed a trend of more than 2 times higher ratio of hearing recovery (> 20 dB) after systemic corticosteroid treatment compared to 4 G/5 G genotype (OR 2.3, 95{\%} CI 0.32 - 16.83, p = 0.39), suggesting a better clinical outcome. Conclusions: The 5 G/5 G genotype of PAI-1may be associated with a reduced risk of SSNHL in the Italian population.",
author = "Cho, {Seong Ho} and Haimei Chen and Kim, {Il Soo} and Chio Yokose and Joseph Kang and David Cho and Chun Cai and Silvia Palma and Micol Busi and Alessandro Martini and Yoo, {Tae J.}",
year = "2012",
month = "9",
day = "10",
doi = "10.1186/1472-6815-12-5",
language = "English (US)",
volume = "12",
journal = "BMC Ear, Nose and Throat Disorders",
issn = "1472-6815",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Association of the 4g/5g polymorphism of plasminogen activator inhibitor-1 gene with sudden sensorineural hearing loss. A case control study

AU - Cho, Seong Ho

AU - Chen, Haimei

AU - Kim, Il Soo

AU - Yokose, Chio

AU - Kang, Joseph

AU - Cho, David

AU - Cai, Chun

AU - Palma, Silvia

AU - Busi, Micol

AU - Martini, Alessandro

AU - Yoo, Tae J.

PY - 2012/9/10

Y1 - 2012/9/10

N2 - Background: The 5 G/5 G genotype of PAI-1 polymorphism is linked to decreased plasminogen activator inhibitor-1 (PAI-1) levels and it has been suggested that lower PAI-1 levels may provide protective effects on inflammation, local microcirculatory disturbance, and fibrotic changes, which are likely associated with development of sudden sensorineural hearing loss (SSNHL). Methods: The association of the 4 G/5 G PAI-1 polymorphism with the development and clinical outcome of SSNHL is evaluated via a case control study. 103 patients with SSNHL and 113 age and sex-matched controls were enrolled at University of Ferrara, Italy and hearing loss outcome was measured at least 3 months after the onset of hearing loss. DNA was isolated from peripheral blood using the QIAamp kit and the 4 G/5 G polymorphism in the -675 promoter region was genotyped with an allele-specific PCR. Genotype distribution was tested in patients and compared to controls by chi-square and odd-ratio analysis. The codominant and recessive models were used for the multiple logistic regression analyses of the PAI-1 gene allele. Results: In this population, 5 G/5 G genotype had a two-time lower frequency in SSNHL patients compared to healthy controls (15.5% vs 30.1%) and was associated with decreased odds compared to 4 G/5 G genotype (OR 0.37, 95% CI 0.19- 0.75, p = 0.005). In addition, the patients with 5 G/5 G genotype showed a trend of more than 2 times higher ratio of hearing recovery (> 20 dB) after systemic corticosteroid treatment compared to 4 G/5 G genotype (OR 2.3, 95% CI 0.32 - 16.83, p = 0.39), suggesting a better clinical outcome. Conclusions: The 5 G/5 G genotype of PAI-1may be associated with a reduced risk of SSNHL in the Italian population.

AB - Background: The 5 G/5 G genotype of PAI-1 polymorphism is linked to decreased plasminogen activator inhibitor-1 (PAI-1) levels and it has been suggested that lower PAI-1 levels may provide protective effects on inflammation, local microcirculatory disturbance, and fibrotic changes, which are likely associated with development of sudden sensorineural hearing loss (SSNHL). Methods: The association of the 4 G/5 G PAI-1 polymorphism with the development and clinical outcome of SSNHL is evaluated via a case control study. 103 patients with SSNHL and 113 age and sex-matched controls were enrolled at University of Ferrara, Italy and hearing loss outcome was measured at least 3 months after the onset of hearing loss. DNA was isolated from peripheral blood using the QIAamp kit and the 4 G/5 G polymorphism in the -675 promoter region was genotyped with an allele-specific PCR. Genotype distribution was tested in patients and compared to controls by chi-square and odd-ratio analysis. The codominant and recessive models were used for the multiple logistic regression analyses of the PAI-1 gene allele. Results: In this population, 5 G/5 G genotype had a two-time lower frequency in SSNHL patients compared to healthy controls (15.5% vs 30.1%) and was associated with decreased odds compared to 4 G/5 G genotype (OR 0.37, 95% CI 0.19- 0.75, p = 0.005). In addition, the patients with 5 G/5 G genotype showed a trend of more than 2 times higher ratio of hearing recovery (> 20 dB) after systemic corticosteroid treatment compared to 4 G/5 G genotype (OR 2.3, 95% CI 0.32 - 16.83, p = 0.39), suggesting a better clinical outcome. Conclusions: The 5 G/5 G genotype of PAI-1may be associated with a reduced risk of SSNHL in the Italian population.

UR - http://www.scopus.com/inward/record.url?scp=84865766646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865766646&partnerID=8YFLogxK

U2 - 10.1186/1472-6815-12-5

DO - 10.1186/1472-6815-12-5

M3 - Article

VL - 12

JO - BMC Ear, Nose and Throat Disorders

JF - BMC Ear, Nose and Throat Disorders

SN - 1472-6815

IS - 1

M1 - 5

ER -