Attenuation of renal ischemia-reperfusion injury by postconditioning involves adenosine receptor and protein kinase C activation

Shady M. Eldaif, Jeremiah Deneve, Ning Ping Wang, Rong Jiang, Mario Mosunjac, Christopher J. Mutrie, Robert A. Guyton, Zhi Qing Zhao, Jakob Vinten-Johansen

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Significant organ injury occurs after transplantation and reflow (i.e., reperfusion injury). Postconditioning (PoC), consisting of alternating periods of reperfusion and re-occlusion at onset of reperfusion, attenuates reperfusion injury in organs including heart and brain. We tested whether PoC attenuates renal ischemia-reperfusion (I/R) injury in the kidney by activating adenosine receptors (AR) and protein kinase C (PKC). The single kidney rat I/R model was used. Groups: (1) sham: time-matched surgical protocol only. In all others, the left renal artery (RA) was occluded for 45 min and reperfused for 24 h. (2) Control: I/R with no intervention at R. All antagonists were administered 5 min before reperfusion. (3) PoC: I/R + four cycles of 45 s of R and 45 s of re-occlusion before full R. (4) PoC + ARi: PoC plus the AR antagonist 8-ρ-(sulfophenyl) theophylline (8-SPT). (5) PoC + PKCi: PoC plus the PKC antagonist chelerythrine (Che). In shams, plasma blood urea nitrogen (BUN mg/dl) at 24 h averaged 23.2 ± 5.3 and creatinine (Cr mg/dl) averaged 1.28 ± 0.2. PoC reduced BUN (87.2 ± 10 in Control vs. 38.8 ± 9, P = 0.001) and Cr (4.2 ± 0.6 in Control vs. 1.5 ± 0.2, P < 0.001). 8-SPT and Che reversed renal protection indices after PoC. I/R increased apoptosis, which was reduced by PoC, which was reversed by 8-SPT and Che. Postconditioning attenuates renal I/R injury by adenosine receptor activation and PKC signaling.

Original languageEnglish (US)
Pages (from-to)217-226
Number of pages10
JournalTransplant International
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2010

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Adenosine Kinase
Purinergic P1 Receptors
Reperfusion Injury
Protein Kinase C
Reperfusion
Kidney
Blood Urea Nitrogen
Ischemia
Theophylline
Purinergic P1 Receptor Antagonists
Renal Artery
Creatinine
Transplantation
Apoptosis
Wounds and Injuries
Brain

All Science Journal Classification (ASJC) codes

  • Transplantation

Cite this

Attenuation of renal ischemia-reperfusion injury by postconditioning involves adenosine receptor and protein kinase C activation. / Eldaif, Shady M.; Deneve, Jeremiah; Wang, Ning Ping; Jiang, Rong; Mosunjac, Mario; Mutrie, Christopher J.; Guyton, Robert A.; Zhao, Zhi Qing; Vinten-Johansen, Jakob.

In: Transplant International, Vol. 23, No. 2, 01.02.2010, p. 217-226.

Research output: Contribution to journalArticle

Eldaif, Shady M. ; Deneve, Jeremiah ; Wang, Ning Ping ; Jiang, Rong ; Mosunjac, Mario ; Mutrie, Christopher J. ; Guyton, Robert A. ; Zhao, Zhi Qing ; Vinten-Johansen, Jakob. / Attenuation of renal ischemia-reperfusion injury by postconditioning involves adenosine receptor and protein kinase C activation. In: Transplant International. 2010 ; Vol. 23, No. 2. pp. 217-226.
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