Atypical SCH23390 binding sites are present on bovine adrenal medullary membranes

Mary K. Dahmer, Susan Senogles

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

D1-selective dopamine receptor agonists inhibit secretagogue-stimulated catecholamine secretion from bovine adrenal chromaffin cells. The purpose of the studies reported here was to use the radiolabeled D1-selective dopamine receptor antagonist, SCH23390, to characterize putative D1-like dopamine receptors responsible for this effect. Characterization of SCH23390 binding sites demonstrated an unusual pharmacological profile inconsistent with classical D1-like receptors. [125I]SCH23390 bound to adrenal medullary membranes was competed for by nonradioactive iodo-SCH23390 (Kd = 490 ± 50 nM), but not by (+)butaclamol. Other classical D1 antagonists had little, if any, effect. Competition with dopamine receptor agonists demonstrated a relative rank order of potency profile characteristic of D1-like dopamine receptors, however, K(i)s were higher than those found in other tissues. The K(i)s for competition of [125I]SCH23390 binding by Cl-APB and SKF38393 (16 and 118 μM, respectively) are nearly identical to the IC50s previously observed for inhibition of secretion (9 and 100 μM, respectively). Combined these data suggest that adrenal medullary membranes contain a novel SCH23390 binding site involved in the inhibition of secretion by D1-selective agonists.

Original languageEnglish (US)
Pages (from-to)321-326
Number of pages6
JournalNeurochemical Research
Volume25
Issue number3
DOIs
StatePublished - Apr 2 2000

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Binding Sites
Membranes
Dopamine D1 Receptors
Dopamine Agonists
Dopamine Receptors
Butaclamol
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Chromaffin Cells
Dopamine Antagonists
Catecholamines
SCH 23390
Pharmacology
Tissue

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

Cite this

Atypical SCH23390 binding sites are present on bovine adrenal medullary membranes. / Dahmer, Mary K.; Senogles, Susan.

In: Neurochemical Research, Vol. 25, No. 3, 02.04.2000, p. 321-326.

Research output: Contribution to journalArticle

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