Augmented transforming growth factor-β1 in gingival crevicular fluid of smokers with chronic periodontitis

Sidney Stein, Bryan E. Green, Mark Scarbecz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Patients who smoke are at increased risk for chronic periodontitis (CP). Also, CP patients who smoke exhibit significantly less reduction of probing depths and gains in clinical attachment compared to non-smokers following periodontal therapy. Several studies suggest that the effects of smoking on the host response may be paramount in regulating the basal systemic inflammatory status and therapeutic outcomes in this cohort. Growth factors, specifically transforming growth factor β1 (TGF-β1), are critical in regulating the wound healing response by controlling cell division, differentiation, and motility. The hypothesis to be tested was that gingival crevicular fluid (GCF) TGF-β1 production was altered in smokers compared to non-smokers with CP. Methods: GCF was collected from smokers and non-smokers with CP, both at baseline and 1 to 2 weeks after initial therapy. GCF volume was determined using an electronic device and TGF-β1 concentration was measured by enzyme-linked immunosorbent assay (ELISA). Results: Smokers exhibited a higher mean concentration of GCF TGF-β1 at baseline compared to non-smokers (P = 0.03). After initial therapy, smokers exhibited significantly less reduction in mean GCF volume compared to non-smokers (P = 0.04). Conclusions: Augmented constitutive production of GCF TGF-β1 in smokers may explain the clinical appearance of fibrotic gingival tissue exhibited by this patient cohort. A diminished reduction in GCF volume in smokers following root instrumentation suggests a chronic inflammatory status in conjunction with an ineffective host response. These findings support the concept that smokers with CP display an altered local inflammatory response after initial therapy, perhaps symptomatic of colonization by residual periodontal pathogens.

Original languageEnglish (US)
Pages (from-to)1619-1626
Number of pages8
JournalJournal of Periodontology
Volume75
Issue number12
DOIs
StatePublished - Dec 1 2004

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Gingival Crevicular Fluid
Chronic Periodontitis
Transforming Growth Factors
Smoke
Therapeutics
Cell Division
Wound Healing
Cell Movement
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Smoking
Enzyme-Linked Immunosorbent Assay
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Augmented transforming growth factor-β1 in gingival crevicular fluid of smokers with chronic periodontitis. / Stein, Sidney; Green, Bryan E.; Scarbecz, Mark.

In: Journal of Periodontology, Vol. 75, No. 12, 01.12.2004, p. 1619-1626.

Research output: Contribution to journalArticle

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