Bacopa monniera ameliorates cognitive impairment and neurodegeneration induced by intracerebroventricular-streptozotocin in rat

behavioral, biochemical, immunohistochemical and histopathological evidences

M. Badruzzaman Khan, Muzamil Ahmad, Saif Ahmad, Tauheed Ishrat, Kumar Vaibhav, Gulrana Khuwaja, Fakhrul Islam

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The standardized extract of Bacopa monniera (BM) is a complex mixture of ingredients with a uniquely wide spectrum of neuropharmacological influences upon the central nervous system including enhanced learning and memory with known antioxidant potential and protection of the brain from oxidative damage. The present study demonstrates the therapeutic efficacy of BM on cognitive impairment and oxidative damage, induced by intracerebroventricular injection of streptozotocin (ICV-STZ) in rat models. Male Wistar rats were pre-treated with BM at a selected dose (30 mg/Kg) given orally for 2 weeks and then were injected bilaterally with ICV-STZ (3 mg/Kg), while sham operated rats were received the same volume of vehicle. Behavioral parameters were subsequently monitored 2 weeks after the surgery using the Morris water maze (MWM) navigation task then were sacrificed for biochemical, immunohistochemical (Cu/Zn-SOD) and histopathological assays. ICV-STZ-infused rats showed significant loss in learning and memory ability, which were significantly improved by BM supplementation. A significant increase in thiobarbituric acid reactive species and a significant decrease in reduced glutathione, antioxidant enzymes in the hippocampus were observed in ICV-STZ rats. Moreover, decrease in Cu/Zn-SOD expression positive cells were observed in the hippocampus of ICV-STZ rats. BM supplementation significantly ameliorated all alterations induced by ICV-STZ in rats. The data suggest that ICV-STZ might cause its neurotoxic effects via the production of free radicals. Our study demonstrates that BM is a powerful antioxidant which prevents cognitive impairment, oxidative damage, and morphological changes in the ICV-STZ-infused rats. Thus, BM may have therapeutic value for the treatment of cognitive impairment.

Original languageEnglish (US)
Pages (from-to)115-127
Number of pages13
JournalMetabolic Brain Disease
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Bacopa
Streptozocin
Rats
Injections
Antioxidants
Hippocampus
Learning
Data storage equipment
Aptitude
Cognitive Dysfunction
Neurology
Complex Mixtures
Surgery
Free Radicals
Glutathione
Wistar Rats
Assays
Brain
Navigation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Bacopa monniera ameliorates cognitive impairment and neurodegeneration induced by intracerebroventricular-streptozotocin in rat : behavioral, biochemical, immunohistochemical and histopathological evidences. / Khan, M. Badruzzaman; Ahmad, Muzamil; Ahmad, Saif; Ishrat, Tauheed; Vaibhav, Kumar; Khuwaja, Gulrana; Islam, Fakhrul.

In: Metabolic Brain Disease, Vol. 30, No. 1, 01.01.2014, p. 115-127.

Research output: Contribution to journalArticle

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