Band 3 is a target protein of spectrin's E2/E3 activity

implication for sickle cell disease and normal red blood cell aging.

T. L. Chang, F. F. Cubillos, D. G. Kakhniashvili, Steven Goodman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have demonstrated that a 125 kDa red blood cell (RBC) membrane protein, being a target of spectrin's E2/E3 activity, is ubiquitinated band 3. This demonstration was based on copurification of this biotinylated-ubiquitinated protein with band 3, immunoprecipitation with band 3 antibody and analysis of proteins associated with strepavidin sepharose by micro liquid chromatography coupled to tandem mass spectrometry (microLC/MS/MS). Further, we demonstrated the presence of ubiquitinated band 3 in vivo by Western blotting of purified band 3 with a monoclonal antibody (FK2) against ubiquitin. The implications of these results for sickle cell disease and RBC aging are discussed.

Original languageEnglish (US)
Pages (from-to)171-177
Number of pages7
JournalCellular and molecular biology (Noisy-le-Grand, France)
Volume50
Issue number2
StatePublished - Jan 1 2004

Fingerprint

Spectrin
Cell Aging
Sickle Cell Anemia
Blood
Aging of materials
Erythrocytes
Cells
Ubiquitinated Proteins
Erythrocyte Anion Exchange Protein 1
Streptavidin
Liquid chromatography
Ubiquitin
Tandem Mass Spectrometry
Immunoprecipitation
Liquid Chromatography
Sepharose
Mass spectrometry
Membrane Proteins
Proteins
Demonstrations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Band 3 is a target protein of spectrin's E2/E3 activity : implication for sickle cell disease and normal red blood cell aging. / Chang, T. L.; Cubillos, F. F.; Kakhniashvili, D. G.; Goodman, Steven.

In: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 50, No. 2, 01.01.2004, p. 171-177.

Research output: Contribution to journalArticle

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