Basal forebrain infusion of HC-3 in rats

Maze learning deficits and neuropathology

B. J. Hurlbut, J. F. Lubar, R. Switzer, John Dougherty, M. L. Eisenstadt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ten adult male Sprague-Dawley rats were infused with hemicholinium (HC-3) using mini-osmotic pumps over a 14 day period through bilateral, chronically implanted cannulae in the nucleus basalis magnocellularis (nbm). Ten matched controls were infused in the same fashion with saline. HC-3 rats receiving implants demonstrated a significant deficit in maze-learning ability compared with individual and group performances before receiving the implants. In saline rats there was no significant difference in maze-learning ability before and after receiving implants. The HC-3 group receiving implants demonstrated a significant deficit in maze-learning ability compared with the saline control group. Serial sections through nbm from control and HC-3 rats indicated that all cannulae were located within infusion range of nbm. In HC-3 subjects, cholinergic cell bodies were destroyed with concurrent degeneration of terminal fields in cortex. Except for cannula insertion damage, the cholinergic neurotransmitter system appeared unharmed in controls. Stains for neuritic plaques and neurofibrillary damage were negative in both groups. The memory deficit in experimental subjects supported by the demonstrated destruction of nbm cholinergic neurons suggests that HC-3 may be useful in the development of an animal model for Alzheimer's Disease.

Original languageEnglish (US)
Pages (from-to)381-393
Number of pages13
JournalPhysiology and Behavior
Volume39
Issue number3
DOIs
StatePublished - Jan 1 1987

Fingerprint

Maze Learning
Basal Nucleus of Meynert
Aptitude
Cholinergic Agents
Hemicholinium 3
Cholinergic Neurons
Amyloid Plaques
Memory Disorders
Neurotransmitter Agents
Sprague Dawley Rats
Alzheimer Disease
Coloring Agents
Animal Models
Control Groups
Basal Forebrain
Neuropathology
Cannula

All Science Journal Classification (ASJC) codes

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Basal forebrain infusion of HC-3 in rats : Maze learning deficits and neuropathology. / Hurlbut, B. J.; Lubar, J. F.; Switzer, R.; Dougherty, John; Eisenstadt, M. L.

In: Physiology and Behavior, Vol. 39, No. 3, 01.01.1987, p. 381-393.

Research output: Contribution to journalArticle

Hurlbut, B. J. ; Lubar, J. F. ; Switzer, R. ; Dougherty, John ; Eisenstadt, M. L. / Basal forebrain infusion of HC-3 in rats : Maze learning deficits and neuropathology. In: Physiology and Behavior. 1987 ; Vol. 39, No. 3. pp. 381-393.
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