Basement Membrane Collagens. Cyanogen Bromide Peptides of the D Chain from Porcine Kidney

Saryu N. Dixit, Andrew Kang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An α chain size collagenous component, designated as the D chain, was isolated from the pepsin digest of porcine kidney cortices. Reduction of the D chain with 2-mercaptoethanol and carboxymethylation with iodoacetic acid resulted in the formation of two smaller components of 75 000 and 15 000 molecular weights. The 75 000 and 15 000 molecular weight components were separated by molecular sieve chromatography on a column of agarose A-5M. The 75 000 molecular weight component was cleaved with CNBr in 70% formic acid. The resulting CNBr peptides were isolated by a combination of ion exchange and molecular sieve chromatography and were characterized for amino acid contents and molecular weights. A total of seven CNBr peptides were obtained, which together accounted for the amino acid content of the intact 75 000 molecular weight component.

Original languageEnglish (US)
Pages (from-to)2692-2696
Number of pages5
JournalBiochemistry
Volume19
Issue number12
DOIs
StatePublished - Jun 1 1980

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Cyanogen Bromide
Basement Membrane
Swine
Collagen
Molecular Weight
Molecular weight
Kidney
Peptides
formic acid
Molecular sieves
Chromatography
Gel Chromatography
Iodoacetic Acid
Amino Acids
Kidney Cortex
Mercaptoethanol
Pepsin A
Ion Exchange
Sepharose
Ion exchange

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Basement Membrane Collagens. Cyanogen Bromide Peptides of the D Chain from Porcine Kidney. / Dixit, Saryu N.; Kang, Andrew.

In: Biochemistry, Vol. 19, No. 12, 01.06.1980, p. 2692-2696.

Research output: Contribution to journalArticle

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