Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts

Geraldine M. McCarthy, Allison M. Macius, Pamela A. Christopherson, Lawrence M. Ryan, Tayebeh Pourmotabbed

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective - Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied. Method - MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media. Results - BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes. Conclusion - BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.

Original languageEnglish (US)
Pages (from-to)56-60
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume57
Issue number1
DOIs
StatePublished - Jan 1 1998

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Matrix Metalloproteinase 9
Metalloproteases
Fibroblasts
Matrix Metalloproteinases
Crystals
Chondrocytes
Gelatinases
Swine
Synovial Fluid
Joints
Messenger RNA
Synovitis
Fluids
Polymerase chain reaction
Transcription
Conditioned Culture Medium
Osteoarthritis
Northern Blotting
Reverse Transcription
Extracellular Matrix

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts. / McCarthy, Geraldine M.; Macius, Allison M.; Christopherson, Pamela A.; Ryan, Lawrence M.; Pourmotabbed, Tayebeh.

In: Annals of the Rheumatic Diseases, Vol. 57, No. 1, 01.01.1998, p. 56-60.

Research output: Contribution to journalArticle

McCarthy, Geraldine M. ; Macius, Allison M. ; Christopherson, Pamela A. ; Ryan, Lawrence M. ; Pourmotabbed, Tayebeh. / Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts. In: Annals of the Rheumatic Diseases. 1998 ; Vol. 57, No. 1. pp. 56-60.
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AU - Ryan, Lawrence M.

AU - Pourmotabbed, Tayebeh

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N2 - Objective - Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied. Method - MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media. Results - BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes. Conclusion - BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.

AB - Objective - Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied. Method - MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media. Results - BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes. Conclusion - BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.

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