Behavioral deficit and decreased GABA receptor functional regulation in the hippocampus of epileptic rats

Effect of bacopa monnieri

Jobin Mathew, Gireesh Gangadharan, Korah Pushpamangalam Kuruvilla, C. S. Paulose

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In the present study, alterations of the General GABA and GABAA receptors in the hippocampus of pilocarpine-induced temporal lobe epileptic rats and the therapeutic application of Bacopa monnieri and its active component Bacoside-A were investigated. Bacopa monnieri (Linn.) is a herbaceous plant belonging to the family Scrophulariaceae. Hippocampus is the major region of the brain belonging to the limbic system and plays an important role in epileptogenesis, memory and learning. Scatchard analysis of [3H]GABA and [3H]bicuculline in the hippocampus of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABAA receptor sub-units such as GABA Aá1, GABAAá5, GABA, and GAD were down regulated (P < 0.001) in the hippocampus of the epileptic rats compared to control. GABA subunit was up regulated. Epileptic rats have deficit in the radial arm and Y maze performance. Bacopa monnieri and Bacoside-A treatment reverses all these changes near to control. Our results suggest that decreased GABA receptors in the hippocampus have an important role in epilepsy associated behavioral deficit, Bacopa monnieri and Bacoside-A have clinical significance in the management of epilepsy.

Original languageEnglish (US)
Pages (from-to)7-16
Number of pages10
JournalNeurochemical Research
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Bacopa
GABA Receptors
Rats
Hippocampus
GABA-A Receptors
gamma-Aminobutyric Acid
Epilepsy
Scrophulariaceae
Pilocarpine
Bicuculline
Limbic System
Amplification
Temporal Lobe
Brain
Real-Time Polymerase Chain Reaction
Data storage equipment
Learning
bacoside A

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Behavioral deficit and decreased GABA receptor functional regulation in the hippocampus of epileptic rats : Effect of bacopa monnieri. / Mathew, Jobin; Gangadharan, Gireesh; Pushpamangalam Kuruvilla, Korah; Paulose, C. S.

In: Neurochemical Research, Vol. 36, No. 1, 01.01.2011, p. 7-16.

Research output: Contribution to journalArticle

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