Binding of collagen α1 chains to human platelets

T. M. Chiang, E. H. Beachey, Andrew Kang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previously the authors have reported that purified α1 chains of type I chick skin collagen induce platelet aggregation. Now a description is given of the immunologic and biochemical evidence that the peptide binds to intact platelets as an early event in the induction of platelet aggregation and the release reaction. Antibody against a1 (I) was obtained by immunizing rabbits with complete Freund's adjuvant mixed with purified α1. Immunofluorescence studies showed that α1(I) treated platelets exhibited strong immunofluorescence. The intensity of fluorescence was markedly decreased by the pretreatment of platelets with α1 CB5 and glucosylgalactosylhydroxylysine. Dose response curves of platelet aggregation induced by α1 and the binding of α1 by washed intact platelets are correlated. The biochemical studies showed that the binding of the α1 chain to washed intact platelets was platelet concentration and temperature dependent, and that it reached a maximum in 10 min. The process was reversible and specific, with an association constant of 1.7 μM. The inhibitor of α1 induced platelet aggregation, glucosylgalactosylhydroxylysine, inhibited the α1 binding. These results suggest that α1(I) chains bind to specific receptor site(s) on platelet membranes to trigger aggregation and the release reaction.

Original languageEnglish (US)
Pages (from-to)405-411
Number of pages7
JournalJournal of Clinical Investigation
Volume59
Issue number3
DOIs
StatePublished - Jan 1 1977

Fingerprint

Collagen
Blood Platelets
Platelet Aggregation
Fluorescent Antibody Technique
Freund's Adjuvant
Fluorescence
Rabbits
Skin
Peptides
Temperature
Membranes
Antibodies
glucosylgalactosylhydroxylysine

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Binding of collagen α1 chains to human platelets. / Chiang, T. M.; Beachey, E. H.; Kang, Andrew.

In: Journal of Clinical Investigation, Vol. 59, No. 3, 01.01.1977, p. 405-411.

Research output: Contribution to journalArticle

Chiang, T. M. ; Beachey, E. H. ; Kang, Andrew. / Binding of collagen α1 chains to human platelets. In: Journal of Clinical Investigation. 1977 ; Vol. 59, No. 3. pp. 405-411.
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