Bioactive factors affect proliferation and phenotypic expression in progenitor and pluripotent stem cells

Henry E. Young, Robert P. Wright, Matthew Mancini, Paul A. Lucas, Charles R. Reagan, Asa C. Black

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Progenitor and pluripotent stem cells reside within connective tissue compartments. They are also present in granulation tissue. This study examined the effects of treating these two cell populations with eight bioactive factors. Cells were assayed for DNA content as a measure of proliferation and for tissue-specific phenotypic markers as measures of lineage progression and lineage commitment. Platelet-derived endothelial growth factor and insulin-like growth factor-II did not induce proliferation in either population. However, dexamethasone, insulin, insulin-like growth factor-I, muscle morphogenetic protein, platelet-derived growth factor-AA, and platelet-derived growth factor-BB stimulated proliferation in one or both cell populations. Platelet-derived growth factor-BB was the most potent stimulator of proliferation in either population. Phenotypic expression markers were induced in the progenitor cells by insulin, insulin-like growth factor-I, insulin-like growth factor-II, dexamethasone, and muscle morphogenetic protein. However, only dexamethasone and muscle morphogenetic protein induced phenotypic expression markers in the pluripotent cells. Platelet-derived endothelial cell growth factor, platelet-derived growth factor-AA, and platelet-derived growth factor-BB did not induce phenotypic expression markers in progenitor or pluripotent cells. This study suggests the potential for using progenitor and pluripotent cells as an in vitro model to ascertain the effects of various bioactive factors on stem cells potentially involved in tissue maintenance and repair.

Original languageEnglish (US)
Pages (from-to)65-75
Number of pages11
JournalWound Repair and Regeneration
Volume6
Issue number1
DOIs
StatePublished - Jan 1 1998

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Pluripotent Stem Cells
Muscle Proteins
Platelet-Derived Growth Factor
Dexamethasone
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Population
Stem Cells
Endothelial Growth Factors
Insulin
Thymidine Phosphorylase
Stem Cell Factor
Granulation Tissue
Connective Tissue
Maintenance
DNA
platelet-derived growth factor BB

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

Bioactive factors affect proliferation and phenotypic expression in progenitor and pluripotent stem cells. / Young, Henry E.; Wright, Robert P.; Mancini, Matthew; Lucas, Paul A.; Reagan, Charles R.; Black, Asa C.

In: Wound Repair and Regeneration, Vol. 6, No. 1, 01.01.1998, p. 65-75.

Research output: Contribution to journalArticle

Young, Henry E. ; Wright, Robert P. ; Mancini, Matthew ; Lucas, Paul A. ; Reagan, Charles R. ; Black, Asa C. / Bioactive factors affect proliferation and phenotypic expression in progenitor and pluripotent stem cells. In: Wound Repair and Regeneration. 1998 ; Vol. 6, No. 1. pp. 65-75.
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