Biochemical differences between dystrophic calcification of cross-linked collagen implants and mineralization during bone induction

Marcel E. Nimni, Sol Bernick, David T. Cheung, Delia C. Ertl, Satoru Nishimoto, Wendelin J. Paule, Carl Salka, Basil S. Strates

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Ectopic calcification of diseased tissues or around prosthetic implants can lead to serious disability. Therefore, calcification of implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen was compared with mineralization induced by demineralized bone matrix (DBM). Whereas implants of DBM accumulated large amounts of calcium and a bone-specific γ-carboxyglutamic acid protein (BGP or osteocalcin) following implantation in both young and older rats, implants of cross-linked pericardium calcified with only traces of BGP. Glutaraldehyde-cross-linked DBM failed to calcify after implantation in 8-month-old rats for 2-16 weeks. Implants of cross-linked type I collagen exhibited small calcific deposits 2 weeks postimplantation but calcium content eventually dropped to levels equal to those of soft tissues as the implants were resorbed. The calcium content of DBM implanted in 1- and 8-month-old rats reached comparable levels after 4 weeks, but the BGP content was approximately twice as high in the younger animals than in the older ones. Glutaraldehyde-cross-linked implants of DBM, tendon, and cartilage calcified significantly in young but not in old animals. This form of dystrophic calcification was associated with only trace amounts of BGP. Alkaline phosphatase activity was high in implants of DBM and undetectable in implants of cross-linked collagenous tissues. These results show that implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen calcify to different extents depending upon their origin and the age of the host, and that the mechanism of dystrophic calcification differs significantly from the process of mineralization associated with bone induction as reflected by alkaline phosphatase activity and BGP accumulation.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalCalcified Tissue International
Volume42
Issue number5
DOIs
StatePublished - Sep 1 1988
Externally publishedYes

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Physiologic Calcification
Bone Matrix
Collagen
Glutaral
Calcium
Alkaline Phosphatase
Bone and Bones
Pericardium
Osteocalcin
Collagen Type I
Tendons
Cartilage
Acids
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Biochemical differences between dystrophic calcification of cross-linked collagen implants and mineralization during bone induction. / Nimni, Marcel E.; Bernick, Sol; Cheung, David T.; Ertl, Delia C.; Nishimoto, Satoru; Paule, Wendelin J.; Salka, Carl; Strates, Basil S.

In: Calcified Tissue International, Vol. 42, No. 5, 01.09.1988, p. 313-320.

Research output: Contribution to journalArticle

Nimni, Marcel E. ; Bernick, Sol ; Cheung, David T. ; Ertl, Delia C. ; Nishimoto, Satoru ; Paule, Wendelin J. ; Salka, Carl ; Strates, Basil S. / Biochemical differences between dystrophic calcification of cross-linked collagen implants and mineralization during bone induction. In: Calcified Tissue International. 1988 ; Vol. 42, No. 5. pp. 313-320.
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AU - Nishimoto, Satoru

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AU - Salka, Carl

AU - Strates, Basil S.

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N2 - Ectopic calcification of diseased tissues or around prosthetic implants can lead to serious disability. Therefore, calcification of implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen was compared with mineralization induced by demineralized bone matrix (DBM). Whereas implants of DBM accumulated large amounts of calcium and a bone-specific γ-carboxyglutamic acid protein (BGP or osteocalcin) following implantation in both young and older rats, implants of cross-linked pericardium calcified with only traces of BGP. Glutaraldehyde-cross-linked DBM failed to calcify after implantation in 8-month-old rats for 2-16 weeks. Implants of cross-linked type I collagen exhibited small calcific deposits 2 weeks postimplantation but calcium content eventually dropped to levels equal to those of soft tissues as the implants were resorbed. The calcium content of DBM implanted in 1- and 8-month-old rats reached comparable levels after 4 weeks, but the BGP content was approximately twice as high in the younger animals than in the older ones. Glutaraldehyde-cross-linked implants of DBM, tendon, and cartilage calcified significantly in young but not in old animals. This form of dystrophic calcification was associated with only trace amounts of BGP. Alkaline phosphatase activity was high in implants of DBM and undetectable in implants of cross-linked collagenous tissues. These results show that implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen calcify to different extents depending upon their origin and the age of the host, and that the mechanism of dystrophic calcification differs significantly from the process of mineralization associated with bone induction as reflected by alkaline phosphatase activity and BGP accumulation.

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