Biofilm Formation of Staphylococcus aureus under Food Heat Processing Conditions

First Report on CML Production within Biofilm

Jian Miao, Shiqi Lin, Thanapop Soteyome, Brian Peters, Yanmei Li, Huishan Chen, Jianyu Su, Lin Li, Bing Li, Zhenbo Xu, Mark E. Shirtliff, Janette M. harro

Research output: Contribution to journalArticle

Abstract

This study aimed to evaluate the Staphylococcus aureus biofilm formation and Nε-carboxymethyl-lysine generation ability under food heat processing conditions including pH (5.0–9.0), temperature (25 °C, 31 °C, 37 °C, 42 °C and 65 °C), NaCl concentration (10%, 15% and 20%, w/v) and glucose concentration (0.5%, 1%, 2%, 3%, 5%, 10%, w/v). S. aureus biofilm genetic character was obtained by PCR detecting atl, ica operon, sasG and agr. Biofilm biomass and metabolic activity were quantified with crystal violet and methyl thiazolyl tetrazolium staining methods. S. aureus biofilm was sensitive to food heat processing conditions with 37 °C, pH 7.0, 2% glucose concentration (w/v) and 10% NaCl concentration (w/v) were favorable conditions. Besides, free and bound Nε-carboxymethyl-lysine level in weak, moderate and strong biofilm were detected by optimized high performance liquid chromatography tandem mass spectrometry. Nε-carboxymethyl-lysine level in S. aureus biofilm possessed a significant gap between strong, moderate and weak biofilm strains. This investigation revealed the biological and chemical hazard of Staphylococcus aureus biofilm to food processing environment.

Original languageEnglish (US)
Article number1312
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Food Handling
Biofilms
Staphylococcus aureus
Hot Temperature
Gentian Violet
Glucose
Operon
Tandem Mass Spectrometry
Biomass
High Pressure Liquid Chromatography
Staining and Labeling
Polymerase Chain Reaction
Temperature

All Science Journal Classification (ASJC) codes

  • General

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Biofilm Formation of Staphylococcus aureus under Food Heat Processing Conditions : First Report on CML Production within Biofilm. / Miao, Jian; Lin, Shiqi; Soteyome, Thanapop; Peters, Brian; Li, Yanmei; Chen, Huishan; Su, Jianyu; Li, Lin; Li, Bing; Xu, Zhenbo; Shirtliff, Mark E.; harro, Janette M.

In: Scientific Reports, Vol. 9, No. 1, 1312, 01.12.2019.

Research output: Contribution to journalArticle

Miao, J, Lin, S, Soteyome, T, Peters, B, Li, Y, Chen, H, Su, J, Li, L, Li, B, Xu, Z, Shirtliff, ME & harro, JM 2019, 'Biofilm Formation of Staphylococcus aureus under Food Heat Processing Conditions: First Report on CML Production within Biofilm', Scientific Reports, vol. 9, no. 1, 1312. https://doi.org/10.1038/s41598-018-35558-2
Miao, Jian ; Lin, Shiqi ; Soteyome, Thanapop ; Peters, Brian ; Li, Yanmei ; Chen, Huishan ; Su, Jianyu ; Li, Lin ; Li, Bing ; Xu, Zhenbo ; Shirtliff, Mark E. ; harro, Janette M. / Biofilm Formation of Staphylococcus aureus under Food Heat Processing Conditions : First Report on CML Production within Biofilm. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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