Bleomycin-induced chromosome breakage in G2 lymphocytes of retinoblastoma patients

Edward Chaum, L. A. Doucette, R. M. Ellsworth, D. H. Abramson, B. G. Haik, F. D. Kitchin, R. S.K. Chaganti

Research output: Contribution to journalArticle

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Abstract

Retinoblastoma (Rb) cells may be more sensitive to X-irradiation than normal cells when assayed for clonogenic survival, suggesting that such sensitivity can be used as a trait for detecting RBI gene carriers. We studied induction of chromosome damage in lymphocytes from patients with Rb by the radiomimetic antineoplastic antibiotic, bleomycin (BL). Lymphocytes were treated in G2 for 4h at four concentrations of the drug (0.0, 0.3. 0.9. and 2.7 μg/ml). and chromosome damage was evaluated. No difference in levels of spontaneous or induced damage was detected among hereditary Rb, non-hereditary Rb, and normal lymphocytes. The role of X-ray treatment in the induction of second tumors in Rb patients is discussed.

Original languageEnglish (US)
Pages (from-to)152-154
Number of pages3
JournalCytogenetic and Genome Research
Volume38
Issue number2
DOIs
StatePublished - Jan 1 1984

Fingerprint

Chromosome Breakage
Retinoblastoma
Bleomycin
Lymphocytes
Antineoplastic Antibiotics
Chromosomes
X-Rays
Survival
Pharmaceutical Preparations
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Chaum, E., Doucette, L. A., Ellsworth, R. M., Abramson, D. H., Haik, B. G., Kitchin, F. D., & Chaganti, R. S. K. (1984). Bleomycin-induced chromosome breakage in G2 lymphocytes of retinoblastoma patients. Cytogenetic and Genome Research, 38(2), 152-154. https://doi.org/10.1159/000132049

Bleomycin-induced chromosome breakage in G2 lymphocytes of retinoblastoma patients. / Chaum, Edward; Doucette, L. A.; Ellsworth, R. M.; Abramson, D. H.; Haik, B. G.; Kitchin, F. D.; Chaganti, R. S.K.

In: Cytogenetic and Genome Research, Vol. 38, No. 2, 01.01.1984, p. 152-154.

Research output: Contribution to journalArticle

Chaum, E, Doucette, LA, Ellsworth, RM, Abramson, DH, Haik, BG, Kitchin, FD & Chaganti, RSK 1984, 'Bleomycin-induced chromosome breakage in G2 lymphocytes of retinoblastoma patients', Cytogenetic and Genome Research, vol. 38, no. 2, pp. 152-154. https://doi.org/10.1159/000132049
Chaum E, Doucette LA, Ellsworth RM, Abramson DH, Haik BG, Kitchin FD et al. Bleomycin-induced chromosome breakage in G2 lymphocytes of retinoblastoma patients. Cytogenetic and Genome Research. 1984 Jan 1;38(2):152-154. https://doi.org/10.1159/000132049
Chaum, Edward ; Doucette, L. A. ; Ellsworth, R. M. ; Abramson, D. H. ; Haik, B. G. ; Kitchin, F. D. ; Chaganti, R. S.K. / Bleomycin-induced chromosome breakage in G2 lymphocytes of retinoblastoma patients. In: Cytogenetic and Genome Research. 1984 ; Vol. 38, No. 2. pp. 152-154.
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