Blood-induced superoxide anion generation on the cerebral cortex of newborn pigs

R. Mirro, W. M. Armstead, J. Mirro, D. W. Busija, Charles Leffler

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Abstract

Pigs were equipped with closed cranial windows to measure superoxide anion generation in response to blood placed on the cerebral cortex. Superoxide dismutase (SOD)-inhibitable nitro blue tetrazolium (NBT) reduction was measured in the presence of 1) artificial cerebrospinal fluid (CSF), 2) homologous nonheparinized blood (blood), and 3) blood after treatment with indomethacin. SOD-inhibitable NBT reduction was increased significantly in the blood group compared with control, whereas piglets pretreated with indomethacin had significantly less SOD-inhibitable NBT reduction. This suggests that superoxide anion is generated by extravascular blood and that this superoxide anion generation can be inhibited by indomethacin. To investigate the cellular origin of superoxide anion generation, SOD-inhibitable NBT reduction was measured during incubation with piglet whole blood or its components in vitro. The SOD-inhibitable NBT reduction of nonheparinized whole blood was 30 ± 5.7 nmol · ml-1 · 20 min-1. This decreased to 2.5 ± 1.3 nmol · ml-1 · 20 min-1 in the presence of indomethacin. SOD-inhibitable NBT reduction of ADP-stimulated platelet-rich plasma was 25.6 ± 2.9 nmol · 400 x 106 cells-1 · 20 min-1 and was decreased significantly (6.8 ± 3.0 nmol · 400 x 106 cells-1 · 20 min-1) in the presence of indomethacin. SOD-inhibitable NBT reduction by granulocytes was significant but unchanged by indomethacin. Similarly, SOD-inhibitable NBT reduction by lymphocytes and monocytes was unaffected by indomethacin. Reduction by the red cell fraction was small. These results suggest that substantial quantities of superoxide anion are generated via the platelet cyclooxygenase pathway of arachidonic acid metabolism.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume257
Issue number5
StatePublished - 1989

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Superoxides
Cerebral Cortex
Superoxide Dismutase
Indomethacin
Swine
Platelet-Rich Plasma
4-anisyltetrazolium blue
Prostaglandin-Endoperoxide Synthases
Blood Group Antigens
Granulocytes
Adenosine Diphosphate
Cerebrospinal Fluid
Monocytes
Blood Platelets
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Physiology

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Blood-induced superoxide anion generation on the cerebral cortex of newborn pigs. / Mirro, R.; Armstead, W. M.; Mirro, J.; Busija, D. W.; Leffler, Charles.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 257, No. 5, 1989.

Research output: Contribution to journalArticle

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