Bluetongue virus -23 stimulates inducible nitric oxide synthase expression and nitric oxide production in mononuclear cells of blood and/or regional lymphoid organs

Channakeshava Sokke Umeshappa, Karam Pal Singh, Roopa Hebbandi Nanjundappa, Rudragouda Channappanavar, Sushila Maan, Narender S. Maan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mononuclear leukocytes of peripheral blood mononuclear cells (PBMCs) and regional lymphoid organs (RLOs) play a critical role in primary BTV replication and subsequent viral dissemination to distant systemic organs. The lesions in animals develop primarily as a result of vascular insult, presumably induced by the activity of viral and/or proinflammatory vasoactive mediators. Hence, the current study was designed in sheep to investigate the responses of potent vasoactivators, inducible nitric oxide synthase (iNOS) and/or nitric oxide (NO) in mononuclear leukocytes of PBMCs and RLOs. The results show that BTV infection of sheep led to enhanced transcription of iNOS in PBMCs and in particular RLOs. The BTV RNAs and/or antigens were readily demonstrable in these mononuclear leukocytes, suggesting the possible role of BTV in iNOS induction. Moreover, upon in vitro infection of PBMCs with BTV-23, iNOS was up-regulated in timedependent fashion and correlated with increased NO production. The results from these in vivo and in vitro studies thus suggest iNOS and NO produced by mononuclear leukocytes may potentially contribute to vascular-related pathology of BT.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalVeterinary Research Communications
Volume36
Issue number4
DOIs
StatePublished - Dec 1 2012

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Bluetongue virus
Nitric Oxide Synthase Type II
Mononuclear Leukocytes
mononuclear leukocytes
blood cells
nitric oxide
Blood Cells
Nitric Oxide
Blood Vessels
Sheep
blood vessels
Infection
sheep
inducible nitric oxide synthase
RNA
Pathology
Antigens
lesions (animal)
infection
in vitro studies

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Bluetongue virus -23 stimulates inducible nitric oxide synthase expression and nitric oxide production in mononuclear cells of blood and/or regional lymphoid organs. / Umeshappa, Channakeshava Sokke; Singh, Karam Pal; Nanjundappa, Roopa Hebbandi; Channappanavar, Rudragouda; Maan, Sushila; Maan, Narender S.

In: Veterinary Research Communications, Vol. 36, No. 4, 01.12.2012, p. 245-250.

Research output: Contribution to journalArticle

Umeshappa, Channakeshava Sokke ; Singh, Karam Pal ; Nanjundappa, Roopa Hebbandi ; Channappanavar, Rudragouda ; Maan, Sushila ; Maan, Narender S. / Bluetongue virus -23 stimulates inducible nitric oxide synthase expression and nitric oxide production in mononuclear cells of blood and/or regional lymphoid organs. In: Veterinary Research Communications. 2012 ; Vol. 36, No. 4. pp. 245-250.
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AU - Singh, Karam Pal

AU - Nanjundappa, Roopa Hebbandi

AU - Channappanavar, Rudragouda

AU - Maan, Sushila

AU - Maan, Narender S.

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