Borrelia burgdorferi BBI39 paralogs, targets of protective immunity, reduce pathogen persistence either in hosts or in the vector

Preeti Singh, Deepshikha Verma, Brian T. Backstedt, Simarjot Kaur, Manish Kumar, Alexis A. Smith, Kavita Sharma, Xiuli Yang, José F. Azevedo, Maria Gomes-Solecki, Ozlem Buyuktanir, Utpal Pal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Borrelia burgdorferi genome harbors several paralogous gene families (pgf) that can encode immunogenic proteins of unknown function. Protein-protein interaction assays using a transmission-blocking vaccine candidate, BBA52, as bait identified an interacting partner in spirochetes-a member of pgf 54, annotated as BBI39. We show that BBI39 is a surface-exposed membrane antigen that is immunogenic during spirochete infection, despite the gene being primarily transcribed in the vector with a transient expression in the host only at tick-bite sites. Immunization of rodents with BBI39, or a diverse paralog, BBI36, or their combination impaired pathogen acquisition by the vector, transmission from ticks to hosts, or induction of disease. High-titer BBI39 immunoglobulin G antibodies, which have borreliacidal properties, could be generated through routine subcutaneous or oral immunization, further highlighting use of BBI39 proteins as novel Lyme disease vaccines that can target pathogens in the host or in ticks.

Original languageEnglish (US)
Pages (from-to)1000-1009
Number of pages10
JournalJournal of Infectious Diseases
Volume215
Issue number6
DOIs
StatePublished - Mar 15 2017

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Borrelia burgdorferi
Immunity
Ticks
Lyme Disease Vaccines
Spirochaetales Infections
Immunization
Proteins
Tick Bites
Genes
Spirochaetales
Rodentia
Vaccines
Immunoglobulin G
Genome
Antigens
Membranes
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Borrelia burgdorferi BBI39 paralogs, targets of protective immunity, reduce pathogen persistence either in hosts or in the vector. / Singh, Preeti; Verma, Deepshikha; Backstedt, Brian T.; Kaur, Simarjot; Kumar, Manish; Smith, Alexis A.; Sharma, Kavita; Yang, Xiuli; Azevedo, José F.; Gomes-Solecki, Maria; Buyuktanir, Ozlem; Pal, Utpal.

In: Journal of Infectious Diseases, Vol. 215, No. 6, 15.03.2017, p. 1000-1009.

Research output: Contribution to journalArticle

Singh, P, Verma, D, Backstedt, BT, Kaur, S, Kumar, M, Smith, AA, Sharma, K, Yang, X, Azevedo, JF, Gomes-Solecki, M, Buyuktanir, O & Pal, U 2017, 'Borrelia burgdorferi BBI39 paralogs, targets of protective immunity, reduce pathogen persistence either in hosts or in the vector', Journal of Infectious Diseases, vol. 215, no. 6, pp. 1000-1009. https://doi.org/10.1093/infdis/jix036
Singh, Preeti ; Verma, Deepshikha ; Backstedt, Brian T. ; Kaur, Simarjot ; Kumar, Manish ; Smith, Alexis A. ; Sharma, Kavita ; Yang, Xiuli ; Azevedo, José F. ; Gomes-Solecki, Maria ; Buyuktanir, Ozlem ; Pal, Utpal. / Borrelia burgdorferi BBI39 paralogs, targets of protective immunity, reduce pathogen persistence either in hosts or in the vector. In: Journal of Infectious Diseases. 2017 ; Vol. 215, No. 6. pp. 1000-1009.
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