Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ élastazy. Ingibirovanie gidroliza élastina, kataliziruemogo leǐkotsitarnoǐ élastazoǐ.

Translated title of the contribution: Bowman-Birk soy inhibitor as an affinity ligand for isolating leukocyte elastase. Inhibition of elastin hydrolysis, catalyzed by leukocyte elastase

T. V. Tikhonova, N. I. Larionova, Inna Gladysheva, N. F. Kazanskaia

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A one-step procedure for human leukocyte elastase purification using an affinity adsorbent based on protein soybean Bowman-Birk proteinase inhibitor has been developed. The leukocyte elastase was purified 70-fold with a 70-100% yield. The enzyme preparations did not contain cathepsin G and displayed a high specific activity. The soybean Bowman-Birk type inhibitor effectively inhibited the elastin hydrolysis by leukocyte elastase both when the enzyme and the inhibitor were simultaneously added to the substrate and after preliminary elastase adsorption on elastin. The inhibitory effect was preserved at high degrees of elastin hydrolysis.

Original languageRussian
Pages (from-to)1669-1676
Number of pages8
JournalBiokhimiya
Volume58
Issue number11
StatePublished - Nov 1 1993

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Leukocyte Elastase
Elastin
Hydrolysis
Ligands
Cathepsin G
Soybean Proteins
Pancreatic Elastase
Enzyme Inhibitors
Adsorbents
Purification
Peptide Hydrolases
Adsorption
Substrates
Enzymes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ élastazy. Ingibirovanie gidroliza élastina, kataliziruemogo leǐkotsitarnoǐ élastazoǐ. / Tikhonova, T. V.; Larionova, N. I.; Gladysheva, Inna; Kazanskaia, N. F.

In: Biokhimiya, Vol. 58, No. 11, 01.11.1993, p. 1669-1676.

Research output: Contribution to journalArticle

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