Brain spectrin(240/235A)

A novel astrocyte specific spectrin isoform

Steven Goodman, Lori L. Lopresti, Beat M. Riederer, Aleksander Sikorski, Ian S. Zagon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have previously demonstrated the existence of two distinct isoforms of spectrin in mammalian brain (23). Brain spectrin(240/235) is found primarily in neuronal axons and presynaptic terminals, and brain spectrin(240/235E) is located in neuronal cell bodies, dendrites and postsynaptic terminals, and oligodendrocytes. These isoforms are thought to play important roles in controlling the early events of synaptic transmission, axonal transport of organelles and vesicles, and lateral mobility of integral membrane proteins. In this study, we have utilized a panel of monoclonal antibodies to identify a novel astrocyte specific isoform(240/235A) with subunits of 240 kDa and 235 kDa in a 1:1 ratio. Double label indirect immunofluorescence has indicated that brain spectrin(240/235A) is distinct from brain spectrin(240/235E). This novel isoform located in the soma and processes of astrocytes may play a role in actin-membrane attachment, cellular architecture, strengthening of the membrane fabric, and translocation of cytoplasmic organelles and vesicles.

Original languageEnglish (US)
Pages (from-to)311-316
Number of pages6
JournalBrain Research Bulletin
Volume23
Issue number4-5
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Spectrin
Astrocytes
Protein Isoforms
Brain
Presynaptic Terminals
Organelles
Cytoplasmic Vesicles
Transport Vesicles
Axonal Transport
Membranes
Oligodendroglia
Carisoprodol
Indirect Fluorescent Antibody Technique
Dendrites
Synaptic Transmission
Actins
Membrane Proteins
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Brain spectrin(240/235A) : A novel astrocyte specific spectrin isoform. / Goodman, Steven; Lopresti, Lori L.; Riederer, Beat M.; Sikorski, Aleksander; Zagon, Ian S.

In: Brain Research Bulletin, Vol. 23, No. 4-5, 01.01.1989, p. 311-316.

Research output: Contribution to journalArticle

Goodman, Steven ; Lopresti, Lori L. ; Riederer, Beat M. ; Sikorski, Aleksander ; Zagon, Ian S. / Brain spectrin(240/235A) : A novel astrocyte specific spectrin isoform. In: Brain Research Bulletin. 1989 ; Vol. 23, No. 4-5. pp. 311-316.
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