Buoyant density determinations on chloroplast DNA in a variegated cytoplasmic mutant of Gossypium hirsutum L

Daniel Kestler, F. R.H. Katterman, J. E. Endrizzi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Chloroplast DNA2 2 cDNA. isolated from normal green tissue of Gossypium hirsutum L. was shown to have buoyant density components of 1.697 and 1.706 gm/cc in neutral CsCl gradients. In cDNA of cytoplasmic mutant yellow and white tissue there was an increase in the 1.706 gm/cc component. In addition, the white tissue cDNA exhibited a unique high buoyant density band of 1.719 gm/cc. The nuclear DNA exhibited a significantly lighter buoyant density (1.692 gm/cc). Ultrastructural studies demonstrated that green tissue chloroplasts had normal grana and thylakoid development, yellow tissue chloroplasts showed only thylakoid development and white tissue chloroplasts appeared as empty vesicles.

Original languageEnglish (US)
Pages (from-to)720-727
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume76
Issue number3
DOIs
StatePublished - Jun 6 1977

Fingerprint

Chloroplast DNA
Gossypium
Chloroplasts
Tissue
Thylakoids
Complementary DNA
DNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Buoyant density determinations on chloroplast DNA in a variegated cytoplasmic mutant of Gossypium hirsutum L. / Kestler, Daniel; Katterman, F. R.H.; Endrizzi, J. E.

In: Biochemical and Biophysical Research Communications, Vol. 76, No. 3, 06.06.1977, p. 720-727.

Research output: Contribution to journalArticle

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