BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex

Ronald Laribee, Nevan J. Krogan, Tiaojiang Xiao, Yoichiro Shibata, Timothy R. Hughes, Jack F. Greenblatt, Brian D. Strahl

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Abstract

Histone-lysine methylation is linked to transcriptional regulation and the control of epigenetic inheritance. Lysine residues can be mono-, di-, or trimethylated, and it has been suggested that each methylation state of a given lysine may impart a unique biological function [1, 2]. In yeast, histone H3 lysine 4 (K4) is mono-, di-, and trimethylated by the Set1 histone methyltransferase [3, 4]. Previous studies show that Set1 associates with RNA polymerase II and demarcates transcriptionally active genes with K4 trimethylation [5]. To determine whether K4 trimethylation might be selectively regulated, we screened a library of yeast deletion mutants associated with transcriptional regulation and chromatin function. We identified BUR2, a cyclin for the Bur1/2 (BUR) cyclin-dependent protein kinase, as a specific regulator of K4 trimethylation [6]. Surprisingly, BUR also regulated H2B monoubiquitylation, whereas other K4 methylation states and H3 lysine 79 (K79) methylation were unaffected. Synthetic genetic array (SGA) and transcription microarray analyses of a BUR2 mutant revealed that BUR is functionally similar to the PAF, Rad6, and Set1 complexes. These data suggest that BUR acts upstream of these factors to control their function. In support, we show that recruitment of the PAF elongation complex to genes is significantly impaired in a BUR2 deletion. Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications.

Original languageEnglish (US)
Pages (from-to)1487-1493
Number of pages7
JournalCurrent Biology
Volume15
Issue number16
DOIs
StatePublished - Aug 23 2005

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Ubiquitination
Methylation
Lysine
Elongation
lysine
phosphotransferases (kinases)
Phosphotransferases
histones
methylation
Histones
Yeast
Histone Code
Genes
Yeasts
Genetic Transcription
yeasts
mutants
Cyclins
cyclin-dependent kinase
Cyclin-Dependent Kinases

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. / Laribee, Ronald; Krogan, Nevan J.; Xiao, Tiaojiang; Shibata, Yoichiro; Hughes, Timothy R.; Greenblatt, Jack F.; Strahl, Brian D.

In: Current Biology, Vol. 15, No. 16, 23.08.2005, p. 1487-1493.

Research output: Contribution to journalArticle

Laribee, Ronald ; Krogan, Nevan J. ; Xiao, Tiaojiang ; Shibata, Yoichiro ; Hughes, Timothy R. ; Greenblatt, Jack F. ; Strahl, Brian D. / BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. In: Current Biology. 2005 ; Vol. 15, No. 16. pp. 1487-1493.
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