Cadmium inhibits EGF-induced DNA synthesis but increases cellular glutathione levels in NRK-49F cells

Yujian Kang, M. Duane Enger

Research output: Contribution to journalArticle

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Abstract

The effects of cadmium (CdCl2) on epidermal growth factor (EGF) induced DNA synthesis and on cellular glutathione (GSH) content in growth-arrested NRK-49F cells were studied. The cadmium effects were compared with those of l-buthionine-(S,R)-sulfoximin (BSO). EGF at a concentration of 10 ng/ml was found to stimulate DNA synthesis (as judged by [3H]thymidine incorporation) in growth-arrested NRK-49F cells. CdCl2 inhibited this EGF-induced DNA synthesis in a dose-dependent fashion. It also increased significantly cellular GSH content in both growth arrested and EGF-stimulated NRK-49F cells. This effect of CdCl2 was contrary to that of BSO, which depleted cellular GSH. Although BSO both inhibited EGF-induced DNA synthesis and decreased cellular GSH content in EGF-stimulated NRK-49F cells, these two BSO effects showed dissimilar dose dependencies. BSO and CdCl2 together inhibited EGF-induced DNA synthesis in NRK-49F cells in an additive fashion. These results demonstrate that cadmium inhibition of EGF-induced DNA synthesis in NRK-49F cells is not due to an effect on cellular GSH content. Both cadmium and BSO inhibit EGF-induced DNA synthesis in NRK-49F cells, but probably through different mechanisms. Although GSH may be involved in regulation of DNA synthesis, BSO-induced inhibition of EGF-stimulated DNA synthesis in NRK-49F cells does not in its dose-dependency correlate with GSH depletion.

Original languageEnglish (US)
Pages (from-to)325-333
Number of pages9
JournalToxicology
Volume66
Issue number3
DOIs
StatePublished - Mar 11 1991
Externally publishedYes

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Cadmium
Epidermal Growth Factor
Glutathione
DNA
Cadmium Chloride
Growth
Thymidine

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Cadmium inhibits EGF-induced DNA synthesis but increases cellular glutathione levels in NRK-49F cells. / Kang, Yujian; Enger, M. Duane.

In: Toxicology, Vol. 66, No. 3, 11.03.1991, p. 325-333.

Research output: Contribution to journalArticle

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